MilkGuard 3 in 1 BTS Combo Test Kit
This kit is used for rapid qualitative analysis of β-lactams, sulfonamides and tetracyclines in raw milk sample.
Beta-lactam and Tetracycline antibiotics are major used antibiotics for the treatment of bacterial infections in dairy cattle, but also for growth promotion and for collective prophylactic treatment.
But using antibiotics for non-therapeutic purposes has led to the development of antibiotic resistant bacteria, which have infiltrated our food system and pose a huge risk to human health.
This kit is based on the specific reaction of antibody-antigen and immunochromatography. β-lactams, sulfonamides and tetracyclines antibiotics in the sample compete for the antibody with the antigen coated on the membrane of the test dipstick. Then after a color reaction, the result can be observed.
There are 4 lines in the dipstick, Control line, Beta-lactams Line, Sulfonamides Line and Tetracylcines Line, which are briefly used as “C”, “T1”,”T2” and “T3”. The test results will depend on the color of these lines. The following diagram describes the result identification.
Negative: Line C, Line T1 ,Line T2 and Line T3 are all red, color of Line T1, Line T2 and Line T3 are all darker than or similar to Line C, indicating the corresponding residue in sample is less than LOD of the kit.
Beta-lactams Positive: Line C is red, color of Line T1 is weaker than Line C, indicating the beta-lactams residue in sample is higher than LOD of the kit. Sulfonamides Positive: Line C is red, color of Line T2 is weaker than Line C, indicating the sulfonamides residue in sample is higher than LOD of the kit.
Tetracyclines Positive: Line T is red, color of Line T3 is weaker than Line C, indicating the tetracyclines residue in sample is higher than LOD of the kit.