ELisa Test Kit of AOZ
About
This kit can be used in quantitative and qualitative analysis of AOZ residue in animal tissues (chicken, cattle, pig, etc), milk , honey and eggs.
The analysis of nitrofuran drugs residue needs to be based on the detection of the tissue bound metabolites of the nitrofuran parent drugs, which include Furazolidone metabolite (AOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) and Nitrofurazone metabolite (SEM).
Compared with chromatographic methods, our kit show considerable advantages regarding sensitivity, detection limit, technical equipment and time requirement.
Kit Components
• Microtiter plate coated with antigen, 96 wells
• Standard solutions(6 bottles,1ml/bottle)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
• Spiking standard control : (1ml/bottle)................................................….100ppb
• Enzyme conjugate concentrate 1.5ml..............................................….….red cap
• Antibody solution concentrated 0.8ml……………………………....…green cap
• Substrate A 7ml……….........................................................…....…..…..white cap
• Substrate B7ml………..............….................................................…..…..red cap
• Stop solution 7ml……………………………………………….………yellow cap
• 20×concentrated wash solution 40ml ……………………….……transparent cap
• 2×concentrated extraction solution 60ml…………………..…………….blue cap
• 2-Nitrobenzaldehyde 15.1mg……………………………………………black cap
Sensitivity, accuracy and precision
Sensitivity: 0.025ppb
Detection limit……………..…………………………0.1ppb
Accuracy:
Animal tissue (muscle and liver)……….…………75±15%
Honey………………………………..………………..90±20%
Egg…………………………………..……………..…90±20%
Milk…………………………………..……………..…90±10%
Precision: CV of the ELISA kit is less than 10%.
Cross Rate
Furazolidone metabolite (AOZ)………………………….……………..100%
Furaltadone metabolite (AMOZ)………………………….……………<0.1%
Nitrofurantoin metabolite (AHD)………………………………………<0.1%
Nitrofurazone metabolite (SEM)…………………….……………...…<0.1%
Furazolidone………………………………………….………….…..…16.3%
Furaltadone……………………………………….………………….…<1%
Nitrofurantoin………………………………………………….…….…<1%
Nitrofurazone…………………………………….……………………..…<1%