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Elisa Test Kit of Ochratoxin A Featured Image
  • Elisa Test Kit of Ochratoxin A
  • Elisa Test Kit of Ochratoxin A
  • Elisa Test Kit of Ochratoxin A
  • Elisa Test Kit of Ochratoxin A

Elisa Test Kit of Ochratoxin A

Short Description:

Ochratoxins are a group of mycotoxins produced by some Aspergillus species (mainly A). Ochratoxin A is known to occur in commodities such as cereals, coffee, dried fruit and red wine. It is considered a human carcinogen and is of special interest as it can be accumulated in the meat of animals. Thus meat and meat products can be contaminated with this toxin. Exposure to ochratoxins through diet can have acute toxicity to mammalian kidneys, and may be carcinogenic.


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About

This kit can be used in quantitative and qualitative analysis of ochratoxin A in feed. It is a new product for drug residue detection based on ELISA technology, which only costs 30min in each operation and can considerably minimize operation errors and work intensity. This kit is based on indirect competitive ELISA technology. The microtiter wells are coated with coupling antigen. Ochratoxin A in sample competes with the antigen coated on the microtiter plate for the a ntibody added . After the addition of enzyme conjugate, TMB substrate is used to show the color. Absorbance of the sample is negatively related to the o chratoxin A residue in it, after comparing with the Standard Curve, multiplied by the dilution factors, Ochratoxin A quantity in the sample can be calculated.

Kit Components

• Microtiter plate with 96 wells coated with antigen

• Standard solutions(6 bottles:1ml/bottle)

0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb

• Enzyme conjugate  7ml………………………………………………………………..………..…..red cap

• Antibody solution   10ml…………………………………………………………………...….…green cap

• Substrate solution A 7ml…………………………………………………………………………white cap

• Substrate Solution B 7ml…………………………………………………………..…………………red cap

• Stop solution 7ml …………………………………………………………….……………………yellow cap

• 20×concentrated Wash solution  40ml………..………………………………….…...…transparent cap

Sensitivity, accuracy and precision

Test Sensitivity: 0.4ppb

Detection limit

Feed………………………………………………….………..…………….…5ppb

Accuracy

Feed………………………………………………………….……….….…90±20%

Precision

Variation coefficient of the ELISA kit is less than 10%.

Cross Rate

Ochratoxin A………………………………………………..………………..100%

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