huahana

ʻO nā kime immunoassay i hoʻopili ʻia e ka enzyme, i ʻike ʻia hoʻi ʻo nā kime ELISA, he ʻenehana bioassay i hoʻokumu ʻia ma ke kumumanaʻo o Enzyme-Linked Immunosorbent Assay (ELISA). Hōʻike nui ʻia kona mau pono ma nā ʻano aʻe:

(1) Wikiwiki: He wikiwiki loa nā kime Enzyme-Linked Immunosorbent Assay, ʻo ka maʻamau he mau minuke a i kekahi mau hola wale nō e loaʻa ai nā hopena. He mea nui kēia no nā maʻi e pono ai ka ʻike wikiwiki, e like me nā maʻi lele koʻikoʻi.
(2) Pololei: Ma muli o ke kikoʻī kiʻekiʻe a me ka ʻike o ka pahu ELISA, pololei loa nā hopena me ka palena haʻahaʻa o ka hewa. ʻO kēia ka mea e hiki ai ke hoʻohana nui ʻia i nā keʻena hoʻokolohua lapaʻau a me nā ʻoihana noiʻi e kōkua i nā kauka i ka ʻike ʻana a me ka nānā ʻana i nā maʻi.
(3) ʻIke kiʻekiʻe: Loaʻa i ka pahu ELISA kahi ʻike kiʻekiʻe loa, hiki ke hōʻea i ka pae pg/mL. ʻO ke ʻano kēia, hiki ke ʻike ʻia nā liʻiliʻi liʻiliʻi o ka mea e hoʻāʻo ʻia, he mea pono loa ia no ka ʻike mua ʻana i ka maʻi.
(4) Kūlana kikoʻī kiʻekiʻe: Loaʻa i nā pahu ELISA ke kūlana kikoʻī kiʻekiʻe a hiki ke hoʻāʻo ʻia e kūʻē i nā antigens a i ʻole nā ​​​​antibodies kikoʻī. Kōkua kēia i ka pale ʻana i ka kuhi hewa a me ka haʻalele ʻana, a hoʻomaikaʻi i ka pololei o ka hōʻailona.
(5) Maʻalahi e hoʻohana: He maʻalahi ka hoʻohana ʻana i nā pahu ELISA a ʻaʻole pono nā lako a i ʻole nā ​​​​ʻano hana paʻakikī. ʻO kēia ka mea e maʻalahi ai ka hoʻohana ʻana i nā ʻano hoʻonohonoho keʻena like ʻole.