Nā kolamu Immunoaffinity Kina no ka ʻike ʻana iā Aflatoxin B1 hale hana a me nā mea hana

Nā kolamu Immunoaffinity no ka ʻike ʻana iā Aflatoxin B1

Wehewehe Pōkole:

Hoʻohana ʻia nā kolamu Kwinbon Aflatoxin B1 ma ka hui pū ʻana me ka HPLC, LC-MS, ELISA test kit. Hiki ke hoʻāʻo nui ʻia ka AFB1 no nā cereals, nā pīnati a me kā lākou huahana, nā aila mea kanu a me nā momona, nā huahana nati, ka soy sauce, ka vīnega, ka lāʻau lapaʻau Kina, nā mea ʻala a me ke kī.

E leka uila mai iā mākou Nā Kuhikuhi No ka Hoʻohana ʻana

Nā kikoʻī huahana

Nā Lepili Huahana

Nā kikoʻī huahana

Pōpoki helu.	KH01104Z
Nā Waiwai	No ka hoʻāʻo ʻana iā Aflatoxin B1
Kahi i hoʻomaka ai	Pekina, Kina
Inoa Lama	ʻO Kwinbon
Ka nui o ka ʻāpana	25 mau hoʻāʻo no kēlā me kēia pahu
Laʻana Noi	Nā meaʻai palaoa, nā pīnati a me kā lākou huahana, nā ʻaila mea kanu a me nā momona, nā huahana nati, ka mea ʻono soy, ka vīnega, ka lāʻau lapaʻau Kina, nā mea ʻala a me ke kī
Ka Waihona	2-30℃
Ola papa	12 mahina
Hoʻouna ʻana	Mahana o ka lumi

Nā Lako a me nā Reagents e Pono ai

Nā lako

Nā mea hoʻohui

Nā lako

----Homogenizer ----Vortex mixer

----ʻŌmole laʻana ----Polo ana: 10ml, 100ml

----Pepa kānana kūlana/Centrifuge ----Kaulike loiloi (inductance: 0.01g)

----Pipette puka: 10ml ----Mea hoʻokomo: 20ml

----ʻŌmole volumetric: 250ml ----ʻŌmole pipette laholio

----Micropipette: 100-1000ul ----ʻAuwai aniani 50ml

----Nā Kānana Microfiber (Whatman, 934-AH, Φ11cm, pōʻai 1.5um)

Nā mea hoʻohui

----Methanol (AR)

----ʻAkika ʻakika (AR)

----Sodium chloride (NACL,AR)

----Wai i hoʻohemo ʻia ka iona

Nā pono huahana

He mea haumia maʻamau ka Aflatoxin B1 i nā ʻano meaʻai like ʻole e like me nā pīnati, ka palaoa pulupulu, ka kulina, a me nā palaoa ʻē aʻe; a me nā meaʻai holoholona. Ua manaʻo ʻia ʻo Aflatoxin B1 ka aflatoxin ʻawahia loa a pili nui ia i ka hepatocellular carcinoma (HCC) i loko o ke kanaka.

Paipai ʻo Wikipedia i nā ʻano hana ʻike aʻe;

Kromatografi papa lahilahi
Ka hoʻāʻo immunosorbent i hoʻopili ʻia e ka enzyme
ʻO ka fluorescence kolamu Immunoaffinity
ʻO ke kolamu Immunoaffinity chromatography wai hana kiʻekiʻe

ʻO ke kolu o ke ʻano hana ʻo Kwinbon Inmmunoaffinity Columns, hoʻohana ia i ka chromatography wai no ka hoʻokaʻawale ʻana, ka hoʻomaʻemaʻe ʻana a i ʻole ka nānā kikoʻī ʻana o Aflatoxin B1. ʻO ka maʻamau, ua hui pū ʻia nā kolamu Kwinbon me HPLC.

He ʻano hana ʻike makua ka loiloi helu HPLC o nā mea ʻawahia fungal. Pili nā chromatography pae mua a me hope. He waiwai kūʻai ka HPLC pae hope, maʻalahi e hana, a he haʻahaʻa ka ʻona solvent. Hoʻoheheʻe ʻia ka hapa nui o nā mea ʻawahia i nā pae mobile polar a laila hoʻokaʻawale ʻia e nā kolamu chromatography non-polar, e hoʻokō ana i nā pono no ka ʻike wikiwiki ʻana i nā mea ʻawahia fungal he nui i loko o ka laʻana waiū. Ke hoʻopili mālie ʻia nei nā mea ʻike i hui pū ʻia ʻo UPLC, me nā modula kaomi kiʻekiʻe a me nā kolamu chromatography liʻiliʻi a me ka nui o ka ʻāpana, hiki ke hoʻopōkole i ka manawa holo laʻana, hoʻomaikaʻi i ka pono hoʻokaʻawale chromatographic, a hoʻokō i ka ʻike kiʻekiʻe.

Me ke ʻano kikoʻī kiʻekiʻe, hiki i nā kolamu Kwinbon Aflatoxin B1 ke hopu i nā molekala i manaʻo ʻia ma ke ʻano maʻemaʻe loa. Ke kahe wikiwiki nei hoʻi nā kolamu Kwinbon, maʻalahi hoʻi e hana. I kēia manawa ke hoʻohana wikiwiki ʻia nei i ka hānai a me ka mahiʻai no ka hoʻopunipuni ʻana i nā mycotoxins.