Nā kolamu Immunoaffinity Kina no ka ʻike ʻana o Ochratoxin A hale hana a me nā mea hana

Nā kolamu Immunoaffinity no ka ʻike ʻana iā Ochratoxin A

Wehewehe Pōkole:

Hoʻohana ʻia nā kolamu Kwinbon Ochratoxin A ma ka hui pū ʻana me ka HPLC, LC-MS, ELISA test kit. Hiki ke hoʻāʻo nui ʻia ka Ochratoxin A no nā palaoa a me nā huahana palaoa, ka mea ʻono soy, ka vīnega, nā huahana mea ʻono, ka waiʻona, ka koko a me ke kofe i kālua ʻia, a pēlā aku.

E leka uila mai iā mākou Nā Kuhikuhi No ka Hoʻohana ʻana

Nā kikoʻī huahana

Nā Lepili Huahana

Nā kikoʻī huahana

Pōpoki helu.	KH00404Z
Nā Waiwai	NoʻOchratoxin A hoʻāʻo ʻana
Kahi i hoʻomaka ai	Pekina, Kina
Inoa Lama	ʻO Kwinbon
Ka nui o ka ʻāpana	25 mau hoʻāʻo no kēlā me kēia pahu
Laʻana Noi	Gnā ua a me nā huahana palaoa, ka mea ʻono soy, ka vīnega, nā huahana mea ʻono, ka waiʻona, ka koko a me ke kofe i kālua ʻia, a pēlā aku
Ka Waihona	2-30℃
Ola papa	12 mahina
Hoʻouna ʻana	Mahana o ka lumi

Nā Lako a me nā Reagents e Pono ai

Nā lako

Nā mea hoʻohui

Nā lako

----Homogenizer ----Vortex mixer

----ʻŌmole laʻana ----Polo ana: 10ml, 100ml

----Pepa kānana kūlana/Centrifuge ----Kaulike loiloi (inductance: 0.01g)

----Pipette puka: 10ml ----Mea hoʻokomo: 20ml

----ʻŌmole volumetric: 250ml ----ʻŌmole pipette laholio

----Micropipette: 100-1000ul ----ʻAuwai aniani 50ml

----Nā Kānana Microfiber (Whatman, 934-AH, Φ11cm, pōʻai 1.5um)

Nā mea hoʻohui

----Methanol (AR)

----ʻAkika ʻakika (AR)

----Sodium chloride (NACL,AR)

----Wai i hoʻohemo ʻia ka iona

Nā pono huahana

E like me ka mea i ʻike ʻia he mycotoxin, ʻo Ochratoxin A (OTA) i hana ʻia e kekahi mau ʻano fungal e like me Aspergillus ochraceus, A. carbonarius, A. niger a me Penicillium verrucosum. Hoʻokumu ka OTA i ka nephrotoxicity a me nā puʻupuʻu renal i nā ʻano holoholona like ʻole; akā naʻe, ʻaʻole i wehewehe maikaʻi ʻia nā hopena olakino kanaka.

ʻO ke kolu o ke ʻano hana ʻo Kwinbon Inmmunoaffinity Columns, hoʻohana ia i ka chromatography wai no ka hoʻokaʻawale ʻana, ka hoʻomaʻemaʻe ʻana a i ʻole ka nānā pono ʻana o Ochratoxin A. ʻO ka maʻamau, ua hui pū ʻia nā kolamu Kwinbon me HPLC.

He ʻano hana ʻike makua ka loiloi helu HPLC o nā mea ʻawahia fungal. Pili nā chromatography pae mua a me hope. He waiwai kūʻai ka HPLC pae hope, maʻalahi e hana, a he haʻahaʻa ka ʻona solvent. Hoʻoheheʻe ʻia ka hapa nui o nā mea ʻawahia i nā pae mobile polar a laila hoʻokaʻawale ʻia e nā kolamu chromatography non-polar, e hoʻokō ana i nā pono no ka ʻike wikiwiki ʻana i nā mea ʻawahia fungal he nui i loko o ka laʻana waiū. Ke hoʻopili mālie ʻia nei nā mea ʻike i hui pū ʻia ʻo UPLC, me nā modula kaomi kiʻekiʻe a me nā kolamu chromatography liʻiliʻi a me ka nui o ka ʻāpana, hiki ke hoʻopōkole i ka manawa holo laʻana, hoʻomaikaʻi i ka pono hoʻokaʻawale chromatographic, a hoʻokō i ka ʻike kiʻekiʻe.

Me ke ʻano kikoʻī kiʻekiʻe, hiki i nā kolamu Kwinbon Ochratoxin A ke hopu i nā molekala i manaʻo ʻia ma ke ʻano maʻemaʻe loa. Ke kahe wikiwiki nei hoʻi nā kolamu Kwinbon, maʻalahi hoʻi e hana. I kēia manawa ke hoʻohana wikiwiki ʻia nei i ka hānai a me ka mahiʻai no ka hoʻopunipuni ʻana i nā mycotoxins.