Nā kolamu Immunoaffinity Kina no ka hale hana ʻike ʻo Zearalenone a me nā mea hana

Nā kolamu Immunoaffinity no ka ʻike ʻana iā Zearalenone

Wehewehe Pōkole:

Hoʻohana ʻia nā kolamu Kwinbon Zearalenone ma ka hui pū ʻana me ka HPLC, LC-MS, ELISA test kit. Hiki ke hoʻāʻo nui ʻia ka AFB1 no nā palaoa a me nā huahana palaoa, ka soy sauce, ka vīnega, nā huahana sauce, ka waiʻona, ka soybeans, ka rapeseed a me nā aila mea kanu, nā mea hānai maka a me nā huahana i hoʻopau ʻia, a pēlā aku.

E leka uila mai iā mākou Nā Kuhikuhi No ka Hoʻohana ʻana

Nā kikoʻī huahana

Nā Lepili Huahana

Nā kikoʻī huahana

Pōpoki helu.	KH00304Z
Nā Waiwai	NoʻO Zearalenonehoʻāʻo ʻana
Kahi i hoʻomaka ai	Pekina, Kina
Inoa Lama	ʻO Kwinbon
Ka nui o ka ʻāpana	25 mau hoʻāʻo no kēlā me kēia pahu
Laʻana Noi	ʻO nā ʻōpala a me nā huahana palaoa, ka mea ʻono soy, ka vīnega, nā huahana mea ʻono, ka waiʻona, ka soybeans, ka rapeseed a me nā aila mea kanu, nā meaʻai maka a me nā huahana i hoʻopau ʻia, a pēlā aku.
Ka Waihona	2-30℃
Ola papa	12 mahina
Hoʻouna ʻana	Mahana o ka lumi

Nā Lako a me nā Reagents e Pono ai

Nā lako

Nā mea hoʻohui

Nā lako

----Homogenizer ----Vortex mixer

----ʻŌmole laʻana ----Polo ana: 10ml, 100ml

----Pepa kānana kūlana/Centrifuge ----Kaulike loiloi (inductance: 0.01g)

----Pipette puka: 10ml ----Mea hoʻokomo: 20ml

----ʻŌmole volumetric: 250ml ----ʻŌmole pipette laholio

----Micropipette: 100-1000ul ----ʻAuwai aniani 50ml

----Nā Kānana Microfiber (Whatman, 934-AH, Φ11cm, pōʻai 1.5um)

Nā mea hoʻohui

----Methanol (AR)

----ʻAkika ʻakika (AR)

----Sodium chloride (NACL,AR)

----Wai i hoʻohemo ʻia ka iona

Nā pono huahana

ʻO Zearalenone (ZEN), i ʻike ʻia hoʻi ʻo RAL a me F-2 mycotoxin, he metabolite estrogenic ikaika i hana ʻia. He kūpaʻa ia i ka wela a loaʻa ma ka honua holoʻokoʻa i loko o kekahi mau mea kanu cereal, e like me ka maize, bale, oats, palaoa, laiki, a me ka sorghum.

ʻO Zearalenone ka mea ʻawahia mua e hoʻopaʻa ai i nā mea loaʻa estrogen, e hoʻoulu ai i ka hānau ʻole ʻana, ka hoʻopau ʻana i ka hāpai keiki a i ʻole nā pilikia hoʻoulu ʻē aʻe, ʻoiai hoʻi i ka puaʻa.

Paipai ʻo Wikipedia i nā ʻano hana ʻike aʻe;

Kromatografi papa lahilahi,
Ka hoʻāʻo immunosorbent i hoʻopili ʻia e ka enzyme
ʻO ka fluorescence kolamu Immunoaffinity
ʻO ke kolamu Immunoaffinity chromatography wai hana kiʻekiʻe

ʻO ke kolu o ke ʻano hana ʻo Kwinbon Inmmunoaffinity Columns, hoʻohana ia i ka chromatography wai no ka hoʻokaʻawale ʻana, ka hoʻomaʻemaʻe ʻana a i ʻole ka nānā kikoʻī ʻana o Zearalenone. ʻO ka maʻamau, ua hui pū ʻia nā kolamu Kwinbon me HPLC.

He ʻano hana ʻike makua ka loiloi helu HPLC o nā mea ʻawahia fungal. Pili nā chromatography pae mua a me hope. He waiwai kūʻai ka HPLC pae hope, maʻalahi e hana, a he haʻahaʻa ka ʻona solvent. Hoʻoheheʻe ʻia ka hapa nui o nā mea ʻawahia i nā pae mobile polar a laila hoʻokaʻawale ʻia e nā kolamu chromatography non-polar, e hoʻokō ana i nā pono no ka ʻike wikiwiki ʻana i nā mea ʻawahia fungal he nui i loko o ka laʻana waiū. Ke hoʻopili mālie ʻia nei nā mea ʻike i hui pū ʻia ʻo UPLC, me nā modula kaomi kiʻekiʻe a me nā kolamu chromatography liʻiliʻi a me ka nui o ka ʻāpana, hiki ke hoʻopōkole i ka manawa holo laʻana, hoʻomaikaʻi i ka pono hoʻokaʻawale chromatographic, a hoʻokō i ka ʻike kiʻekiʻe.

Me ke ʻano kikoʻī kiʻekiʻe, hiki i nā kolamu Kwinbon Zearalenone ke hopu i nā molekala i manaʻo ʻia ma ke ʻano maʻemaʻe loa. Ke kahe wikiwiki nei hoʻi nā kolamu Kwinbon, maʻalahi hoʻi e hana. I kēia manawa ke hoʻohana wikiwiki ʻia nei i ka hānai a me ka mahiʻai palaoa no ka hoʻopunipuni ʻana i nā mycotoxins.