huahana

Hoʻololi koke ʻia nā Nitrofurans i loko o ke kino, a ʻo kā lākou metabolites i hui pū ʻia me nā ʻiʻo e mau ana no ka manawa lōʻihi, no laila e hilinaʻi ka nānā ʻana i ke koena o kēia mau lāʻau i ka ʻike ʻana i kā lākou metabolites, me ka furazolidone metabolite (AOZ), furaltadone metabolite (AMOZ), nitrofurantoin metabolite (AHD) a me ka nitrofurazone metabolite (SEM).

ʻO nā kime Kwinbon Competitive Enzyme Immunoassay, i ʻike ʻia hoʻi ʻo nā kime Elisa, he ʻenehana bioassay i hoʻokumu ʻia ma ke kumumanaʻo o Enzyme-Linked Immunosorbent Assay (ELISA). Hōʻike nui ʻia kona mau pono ma nā ʻano aʻe:

 (1) Ka wikiwikiʻO ka maʻamau, hoʻohana nā keʻena hoʻokolohua i ka LC-MS a me LC-MS/MS e ʻike i ka metabolite nitrofurazone. Eia nō naʻe, ʻo ka hoʻāʻo Kwinbon ELISA, kahi i ʻoi aku ka pololei o ka antibody kikoʻī o ka derivative SEM, ʻike, a maʻalahi hoʻi e hana. ʻO ka manawa hoʻāʻo o kēia pahu he 1.5h wale nō, he mea kūpono loa ia e loaʻa ai nā hopena. He mea nui kēia no ka ʻike wikiwiki ʻana a me ka hōʻemi ʻana i ka ikaika o ka hana.

(2) PololeiMa muli o ke kiʻekiʻe o ke kikoʻī a me ka ʻike o ka pahu Kwinbon SEM Elisa, pololei loa nā hopena me ka palena haʻahaʻa o ka hewa. Hiki i kēia ke hoʻohana nui ʻia i nā keʻena hoʻokolohua lapaʻau a me nā ʻoihana noiʻi e kōkua i nā mahi lawaiʻa a me nā mea hoʻopuka huahana wai i ka ʻike ʻana a me ka nānā ʻana i nā koena lāʻau lapaʻau SEM veterinary i nā huahana wai.

(3) Kūlana kiʻekiʻe: Loaʻa i ka pahu Kwinbon SEM Elisa ke ʻano kikoʻī kiʻekiʻe a hiki ke hoʻāʻo ʻia e kūʻē i ka antibody kikoʻī. ʻO ka hopena kea o SEM a me kāna metabolite he 100%. Hōʻike ka hopena Corss i ka emi ʻana o 0.1% o AOZ, AMOZ, AHD, CAP a me kā lākou metabolites, Kōkua ia i ka pale ʻana i ka misdiagnosis a me ka haʻalele ʻana.