malonda

Monga momwe zimadziwikira kuti mycotoxin ndi yodziwika bwino, Ochratoxin A (OTA) yopangidwa ndi mitundu ingapo ya bowa kuphatikizapo Aspergillus ochraceus, A. carbonarius, A. niger ndi Penicillium verrucosum. OTA imayambitsa poizoni wa nephrotoxicity ndi zotupa za impso m'mitundu yosiyanasiyana ya nyama; komabe, zotsatira za thanzi la anthu sizidziwika bwino.

Kwinbon Inmmunoaffinity Columns ndi njira yachitatu, imagwiritsa ntchito madzi chromatography polekanitsa, kuyeretsa kapena kusanthula kwapadera kwa Ochratoxin A. Nthawi zambiri mizati ya Kwinbon imaphatikizidwa ndi HPLC.

Kusanthula kwa HPLC kwa poizoni wa bowa ndi njira yodziwira bwino. Chromatography ya kutsogolo ndi kumbuyo imagwiritsidwa ntchito. HPLC ya reverse phase ndi yotsika mtengo, yosavuta kugwiritsa ntchito, ndipo ili ndi poizoni wochepa wa solvent. Ma poizoni ambiri amasungunuka m'magawo oyenda a polar kenako amalekanitsidwa ndi ma column a non-polar chromatography, zomwe zimakwaniritsa zosowa zodziwira mwachangu poizoni wambiri wa bowa mu zitsanzo za mkaka. Zipangizo zolumikizirana za UPLC zikugwiritsidwa ntchito pang'onopang'ono, ndi ma module okwera kwambiri komanso ma column ang'onoang'ono a chromatography a kukula ndi tinthu tating'ono, zomwe zimatha kufupikitsa nthawi yogwira ntchito ya zitsanzo, kukonza magwiridwe antchito a chromatography, ndikukwaniritsa kukhudzidwa kwakukulu.

Ndi luso lapadera, Kwinbon Ochratoxin A columns imatha kugwira mamolekyu omwe akufuna kukhala oyera kwambiri. Komanso Kwinbon columns imayenda mwachangu, mosavuta kugwira ntchito. Tsopano ikugwiritsidwa ntchito mwachangu komanso mofala m'munda wa chakudya ndi tirigu powononga mycotoxins.