China Immunoaffinity mizati kwa Vomitoxin kudziwika fakitale ndi opanga

Mipingo ya Immunoaffinity yozindikira Vomitoxin

Kufotokozera Kwachidule:

Zipilala za Kwinbon Vomitoxin zimagwiritsidwa ntchito pophatikiza ndi HPLC, LC-MS, ELISA test kit.Kungakhale kuyesa kuchuluka kwa AFB1 kwa chimanga, mtedza ndi zinthu zawo, mafuta a masamba ndi mafuta, zinthu za mtedza, msuzi wa soya, viniga, mankhwala achi China, zokometsera ndi tiyi.

Titumizireni imelo Malangizo Ogwiritsa Ntchito

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Mafotokozedwe azinthu

Mphaka no.	KH00203Z
Katundu	Kwa kuyesa kwa Vomitoxin
Malo Ochokera	Beijing, China
Dzina la Brand	Kwinbon
Kukula kwa Unit	25 mayesero pa bokosi
Chitsanzo Chogwiritsira Ntchito	Mtedza, mtedza ndi zinthu zawo, mafuta a masamba ndi mafuta, zinthu za mtedza, msuzi wa soya, viniga, mankhwala achi China, zonunkhira ndi tiyi.
Kusungirako	2-30 ℃
Alumali moyo	12 miyezi
Kutumiza	Kutentha kwachipinda

Zida & Reagents Zofunika

Zida

Ma reagents

Zida

----Homogenizer ---- Vortex chosakanizira

----Botolo lachitsanzo ----Silinda yoyezera: 10ml, 100ml

----Pepala losefera labwino/Centrifuge -----Kuwerengera bwino (kulowetsa: 0.01g)

----Pipette yomaliza maphunziro: 10ml ----Injector: 20ml

----Botolo la volumetric: 250ml ----Babu la rabara la pipette

----Micropipette: 100-1000ul ----Faneli yagalasi 50ml

----Zosefera za Microfiber (Whatman, 934-AH, Φ11cm, 1.5um circle)

Ma reagents

Methanol (AR)

----Acetic acid (AR)

----Sodium chloride (NACL,AR)

----Madzi oyeretsedwa

Ubwino wa mankhwala

Vomitoxin, yomwe imadziwikanso kuti deoxynivalenol (DON), ndi epoxy-sesquiterpenoid. Amapezeka makamaka mumbewu monga tirigu, balere, oats, rye, chimanga, ndipo nthawi zambiri mu mpunga, manyuchi, ndi triticale, zomwe zimayambitsa kusanza ndi anorexia mwa nyama ndipo zomwe zingayambitse nseru ndi kusanza mwa anthu.

Wikipedia imalimbikitsa njira zotsatirazi zopezera matenda;

Thin layer chromatography,
Kuyesa kwa immunosorbent kumalumikizidwa ndi enzyme
Immunoaffinity column fluorescence
Immunoaffinity column high performance liquid chromatography

Kwinbon Inmmunoaffinity Columns ndi njira yachitatu, imagwiritsa ntchito madzi chromatography polekanitsa, kuyeretsa kapena kusanthula kwapadera kwa Vomitoxin. Nthawi zambiri Kwinbon columns imaphatikizidwa ndi HPLC.

Kusanthula kwa HPLC kwa poizoni wa bowa ndi njira yodziwira bwino. Chromatography ya kutsogolo ndi kumbuyo imagwiritsidwa ntchito. HPLC ya reverse phase ndi yotsika mtengo, yosavuta kugwiritsa ntchito, ndipo ili ndi poizoni wochepa wa solvent. Ma poizoni ambiri amasungunuka m'magawo oyenda a polar kenako amalekanitsidwa ndi ma column a non-polar chromatography, zomwe zimakwaniritsa zosowa zodziwira mwachangu poizoni wambiri wa bowa mu zitsanzo za mkaka. Zipangizo zolumikizirana za UPLC zikugwiritsidwa ntchito pang'onopang'ono, ndi ma module okwera kwambiri komanso ma column ang'onoang'ono a chromatography a kukula ndi tinthu tating'ono, zomwe zimatha kufupikitsa nthawi yogwira ntchito ya zitsanzo, kukonza magwiridwe antchito a chromatography, ndikukwaniritsa kukhudzidwa kwakukulu.

Ndi kutsimikizika kwakukulu, mizati ya Kwinbon Vomitoxin imatha kugwira mamolekyu omwe akuwatsata mumkhalidwe woyera kwambiri. Komanso mizati ya Kwinbon imayenda mwachangu, yosavuta kugwira ntchito. Tsopano ikugwiritsidwa ntchito mwachangu komanso mofala m'munda wa chakudya ndi tirigu pachinyengo cha mycotoxins.