malonda

Ma nitrofurans amasinthidwa mwachangu kwambiri m'thupi, ndipo ma metabolites awo ophatikizidwa ndi minofu amakhalapo kwa nthawi yayitali, kotero kusanthula zotsalira za mankhwalawa kumadalira kuzindikira kwa ma metabolites awo, kuphatikizapo furazolidone metabolite (AOZ), furaltadone metabolite (AMOZ), nitrofurantoin metabolite (AHD) ndi nitrofurazone metabolite (SEM).

Ma kit a Kwinbon Competitive Enzyme Immunoassay, omwe amadziwikanso kuti ma kit a Elisa, ndi ukadaulo woyesera zinthu pogwiritsa ntchito Enzyme-Linked Immunosorbent Assay (ELISA). Ubwino wake umaonekera kwambiri m'mbali zotsatirazi:

 (1) Kuthamanga: Kawirikawiri ma lab amagwiritsa ntchito LC-MS ndi LC-MS/MS kuti azindikire metabolite ya nitrofurazone. Komabe mayeso a Kwinbon ELISA, momwe ma antibody enieni a SEM derivative ndi olondola, osavuta kugwiritsa ntchito, komanso osavuta kugwiritsa ntchito. Nthawi yoyesera ya kit iyi ndi maola 1.5 okha, zomwe zimathandiza kwambiri kupeza zotsatira. Izi ndizofunikira kuti matenda azitha kudziwika mwachangu komanso kuchepetsa mphamvu ya ntchito.

(2) Kulondola: Chifukwa cha kulunjika kwakukulu komanso kukhudzidwa kwa zida za Kwinbon SEM Elisa, zotsatira zake ndi zolondola kwambiri komanso zolakwika zochepa. Izi zimathandiza kuti zigwiritsidwe ntchito kwambiri m'ma laboratories azachipatala ndi mabungwe ofufuza kuti zithandize minda ya usodzi ndi ogulitsa zinthu zam'madzi pofufuza ndi kuyang'anira zotsalira za mankhwala a SEM m'zinthu zam'madzi.

(3) Kufotokoza mwapadera kwambiri: Kit ya Kwinbon SEM Elisa ili ndi mawonekedwe apadera ndipo imatha kuyesedwa motsutsana ndi ma antibodies enaake. Kuyanjana kwa SEM ndi metabolite yake ndi 100%. Kuyanjana kwa Corss kumasonyeza zochepa 0.1% za AOZ, AMOZ, AHD, CAP ndi ma metabolites awo, Zimathandiza kupewa matenda olakwika ndi kuchotsedwa.