ngwaahịa

Ngwa immunoassay nke enzyme jikọtara, nke a makwaara dị ka ngwa ELISA, bụ teknụzụ bioassay nke dabere na ụkpụrụ nke Enzyme-Linked Immunosorbent Assay (ELISA). Uru ya na-egosipụtakarị n'akụkụ ndị a:

(1) Ọsọ ọsọ: Ngwa nyocha nke Enzyme-Linked Immunosorbent na-adị ngwa ngwa, na-ewekarị nkeji ole na ole ruo awa ole na ole iji nweta nsonaazụ. Nke a dị mkpa maka ọrịa ndị chọrọ nchọpụta ngwa ngwa, dịka ọrịa ndị na-efe efe dị oke njọ.
(2) Izi ezi: N'ihi nkọwapụta na mmetụta dị elu nke ngwa ELISA, nsonaazụ ya ziri ezi nke ukwuu yana obere njehie. Nke a na-eme ka e jiri ya mee ihe nke ọma n'ụlọ nyocha ahụike na ụlọ nyocha iji nyere ndị dọkịta aka n'ịchọpụta na inyocha ọrịa.
(3) Nnukwu mmetụta uche: Ngwa ELISA nwere nnukwu mmetụta uche, nke nwere ike iru ọkwa pg/mL. Nke a pụtara na enwere ike ịchọpụta ọbụna obere ihe a ga-anwale, nke bara ezigbo uru maka nchọpụta ọrịa n'oge.
(4) Nkọwapụta dị elu: Ngwa ELISA nwere nkọwapụta dị elu ma enwere ike ịnwale ya megide antigens ma ọ bụ antibodies ụfọdụ. Nke a na-enyere aka izere nchọpụta na-ezighi ezi na iwepụ ihe, ma melite izi ezi nke nchọpụta ahụ.
(5) Mfe iji: Ngwa ELISA dị mfe iji, ọ chọghịkwa ngwa ma ọ bụ usoro dị mgbagwoju anya. Nke a na-eme ka ọ dị mfe iji n'ọtụtụ ebe nyocha.