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Ngwa ule Elisa nke CAP
Enwere ike iji Kwinbon ngwa a na nyocha ọnụọgụ na qualitative nke CAP residue na ngwaahịa mmiri mmiri shrimp wdg.
Emebere ya iji chọpụta chloramphenicol dabere na isi nke “na asọmpi kpọmkwem” enzyme immunoassay.A na-ekpuchi olulu mmiri microtiter na antigen njikọ.Chloramphenicol dị na nlele ahụ na-asọ mpi antigen mkpuchi maka ijikọ ọnụ ọgụgụ mgbochi mgbochi agbakwunyere.Mgbe mgbakwunye na njikere iji TMB sub strate a na-atụ mgbaàmà na onye na-agụ ELISA.Mmụba a na-agbanwe agbanwe na mkpokọta chloramphenicol dị na nlele.
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Asọmpi Enzyme Immunoassay Kit maka Quantitative Analysis of Tylosin
Tylosin bụ ọgwụ nje macrolide, nke a na-ejikarị dị ka antibacterial na anti-mycoplasma.E guzobela MRL siri ike ebe ọ bụ na ọgwụ a nwere ike ibute mmetụta dị njọ na ụfọdụ otu.
Ngwa a bụ ngwaahịa ọhụrụ dabere na teknụzụ ELISA, nke dị ngwa, dị mfe, ziri ezi ma nwee mmetụta ma e jiri ya tụnyere nyocha ngwa ngwa na-achọ naanị awa 1.5 n'otu ọrụ, ọ nwere ike belata njehie ọrụ yana ike ọrụ.
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Ngwa Enzyme Immunoassay nke asọmpi maka nyocha ọnụọgụ nke Flumequine
Flumequine bụ otu n'ime ọgwụ antibacterial quinolone, nke a na-eji dị ka ihe mgbochi dị oke mkpa na ọgwụgwọ anụ ụlọ na ngwaahịa mmiri maka nnukwu ụdịdị dị iche iche, arụmọrụ dị elu, obere nsị na ntinye anụ ahụ siri ike.A na-ejikwa ya maka ọgwụgwọ ọrịa, mgbochi na nkwalite uto.N'ihi na ọ nwere ike ibute nguzogide ọgwụ na ike ọrịa carcinogenicity, oke oke nke dị n'ime anụ ahụ ka edepụtara na EU, Japan (oke oke bụ 100ppb na EU).
Ka ọ dị ugbu a, spectrofluorometer, ELISA na HPLC bụ ụzọ isi chọpụta ihe fọdụrụ na flumequine, ELISA abụwokwa usoro a na-eme kwa oge maka mmetụta dị elu na ịrụ ọrụ dị mfe.
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Ngwa ule Elisa nke AOZ
Enwere ike iji ngwa a mee ihe na nyocha nke ọnụọgụ na nyocha nke AOZ fọdụrụnụ na anụ anụ anụ (ọkụkọ, ehi, ezi, wdg), mmiri ara ehi , mmanụ aṅụ na akwa.
Nyocha nke ihe fọdụrụ na ọgwụ nitrofuran kwesịrị ịdabere na nchọpụta nke anụ ahụ na-ejikọta metabolites nke ọgwụ nne na nna nitrofuran, nke gụnyere Furazolidone metabolite (AOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) na Nitrofurazone metabolite (SEM).
Tụnyere usoro chromatographic, ngwa anyị na-egosi uru dị ukwuu gbasara uche, oke nchọpụta, akụrụngwa teknụzụ na oge achọrọ. -
Ngwa ule Elisa nke Ochratoxin A
Enwere ike iji ngwa a na nyocha ọnụọgụ na qualitative nke ochratoxin A na nri.Ọ bụ ngwaahịa ọhụrụ maka nchọpụta ihe fọdụrụ ọgwụ dabere na teknụzụ ELISA, nke na-efu naanị 30min n'ọrụ ọ bụla ma nwee ike ibelata mperi ọrụ yana ike ọrụ.Ngwa a dabere na teknụzụ ELISA asọmpi na-apụtaghị ìhè.A na-ekpuchi olulu mmiri microtiter na antigen njikọ.Ochratoxin A na sample na-asọ mpi na antigen mkpuchi na efere microtiter maka ntibody agbakwunyere.Mgbe mgbakwunye nke enzyme conjugate, a na-eji mkpụrụ TMB gosi agba.Mwepu nke sample bụ ihe na-adịghị mma metụtara o chratoxin A n'ime ya, ka e jiri ya tụnyere Standard Curve, mụbaa site dilution ihe, Ochratoxin A quantity na sample nwere ike gbakọọ.
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Ngwa ule Elisa nke Aflatoxin B1
Aflatoxin B1 bụ kemịkalụ na-egbu egbu nke na-emerụ ọka, ọka na ahụekere, wdg. E guzobela oke ihe fọdụrụ maka aflatoxin B1 na nri anụmanụ, nri na ihe nlele ndị ọzọ.Ngwaahịa a dabere na ELISA asọmpi na-apụtaghị ìhè, nke dị ngwa ngwa, nke ziri ezi ma nwee mmetụta ma e jiri ya tụnyere nyocha ngwa ngwa.Ọ chọrọ naanị 45min n'otu ọrụ, nke nwere ike ibelata njehie ọrụ yana ike ọrụ.
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Ngwa ule Elisa nke AMOZ
Enwere ike iji ngwa a mee ihe na nyocha nke quantitative na qualitative nke AMOZ fọdụrụnụ na ngwaahịa mmiri (azụ na oporo), wdg Enzyme immunoassays, ma e jiri ya tụnyere usoro chromatographic, na-egosi uru dị ukwuu banyere mmetụta uche, njedebe nchọpụta, akụrụngwa teknụzụ na oge achọrọ.
Emebere ngwa a iji chọpụta AMOZ dabere na ụkpụrụ nke immunoassay enzyme asọmpi na-apụtaghị ìhè.A na-ejikọta olulu mmiri microtiter na njide BSA
antigen.AMOZ na sample na-asọmpi na antigen mkpuchi na efere microtiter maka mgbochi agbakwunyere.Mgbe mgbakwunye nke enzyme conjugate, a na-eji mkpụrụ chromogenic eme ihe wee tụọ mgbama site na spectrophotometer.Mmụba a na-agbanwe agbanwe na ntinye AM OZ na nlele.
