ngwaahịa

A na-eme ka nitrofurans gbapụta n'ime ahụ ngwa ngwa, njikọta nke metabolites ha na anụ ahụ ga-adịru ogologo oge, yabụ nyocha ihe fọdụrụ nke ọgwụ ndị a ga-adabere na nchọpụta nke metabolites ha, gụnyere furazolidone metabolite (AOZ), furaltadone metabolite (AMOZ), nitrofurantoin metabolite (AHD) na nitrofurazone metabolite (SEM).

Ngwa Kwinbon Competitive Enzyme Immunoassay, nke a makwaara dị ka Elisa kits, bụ teknụzụ bioassay dabere na ụkpụrụ nke Enzyme-Linked Immunosorbent Assay (ELISA). Uru ya na-egosipụtakarị n'akụkụ ndị a:

 (1) Ọsọ ọsọ: Ọtụtụ mgbe, ụlọ nyocha na-eji LC-MS na LC-MS/MS achọpụta metabolite nitrofurazone. Agbanyeghị, ule Kwinbon ELISA, nke ọgwụ mgbochi SEM kpọmkwem ziri ezi, dị nro, ma dị mfe ịrụ ọrụ. Oge nnwale nke ngwa a bụ naanị awa 1.5, nke dị irè nke ukwuu iji nweta nsonaazụ. Nke a dị mkpa maka nchọpụta ngwa ngwa na ibelata ike ọrụ.

(2) Izi ezi: N'ihi nkọwapụta na mmetụta dị elu nke ngwa Kwinbon SEM Elisa, nsonaazụ ya ziri ezi nke ukwuu yana obere njehie. Nke a na-eme ka e jiri ya mee ihe nke ọma na ụlọ nyocha ahụike na ụlọ nyocha iji nyere ndị na-ebupụ ngwaahịa mmiri aka n'ịchọpụta na nlekota nke ihe fọdụrụ na ọgwụ anụmanụ nke SEM na ngwaahịa mmiri.

(3) Nkọwapụta dị elu: Ngwa Kwinbon SEM Elisa nwere nkọwapụta dị elu ma enwere ike ịnwale ya megide ihe mgbochi ụfọdụ. Mmeghachi omume nke SEM na metabolite ya bụ 100%. Mmeghachi omume Corss na-egosi obere 0.1% nke AOZ, AMOZ, AHD, CAP na metabolites ha. Ọ na-enyere aka izere nchọpụta na-ezighi ezi na iwepụ ihe.