ngā purongo

MilkGuard®16-i roto-i-te-1Kete Whakamātautau TereI whakarewahia: Mata 16PaturopiNgā Akoranga i roto i te Miraka Mata i roto i te 9 Miniti

Ngā Painga Matua

Tirotiro Whānui-Teitei

Ka kitea i te wā kotahi ngā rōpū paturopi e 4 puta noa i ngā toenga rongoā e 16:
• Ngā Sulfonamide (SABT)
• Ngā Kuinorona (TEQL)
• Ngā Aminoglycosides (SNKG)
• Ngā Macrolide (SMCF)

Ngā āheinga kimi matua:

Sulfadiazine (8 μg/L), Sulfadimidine (15-20 μg/L)

Florfenicol (0.15 μg/L, EU MRL ≤100 μg/L)

Monensin (5 μg/L), Colistin (10 μg/L)

E tutuki ana i ngā herenga ture a Haina GB 31650 me EU EC/37/2010

Hangarau Pānui-Rua

Whakamārama tirohangaWhakatauritea te rārangi whakamātautau (rārangi-T) me te rārangi whakahaere (rārangi-C) mō te waeatanga kino/pai inamata

Te ine i ngā taputapuKa aunoa te tātari koura colloidal i te pūrongo raraunga me te manatoko puranga waehere QR

Rerenga Mahi Whakangawari

Te tukanga katoa e 9 meneti te roa: 3-meneti te whakatō tauira + 6-meneti te whakatō chromatography

Kāri 4-rīpene whakauruTe kimi whakarara i te taahiraa kotahi

Tauira iti rawa200 μL miraka mata, kāore e hiahiatia he maimoatanga i mua

Kawa Whakapūmau

1. TE WHAKARITE I TE TAUIRA:

- Whakaōritehia te miraka ki te pāmahana rūma (kua rite te ranunga)

- Whakamahanahia te incubator whakarewa ki te 45°C (E taunakitia ana: Kwinbon Mini-T4)

2. TE TIKANGA WHAKAMĀTAUTAU:

- Tāpirihia te 200μL miraka ki te microwell, whakaranua mā te pipetting 5x

- Whakauruhia te kāri whakamātautau (kua rumakina katoatia te papa mimiti)

3. TE WHAKAMĀRAMA (I ROTO I TE 2 MINITI):

- ĀTAAO:

Tōraro: Te kaha o te rārangi-T ≥ te rārangi-C (ngā toenga < LOD)

Pai: Rārangi-T < Rārangi-C, kāore rānei e kitea (ngā toenga ≥ LOD)

- TAPUTAPU:

Whakauruhia te kāri ki te tātaritanga → Matawaihia te waehere QR → Pūrongo tauanga aunoa

Uara Ahumahi

Te whai huatanga o te utuKa whakaiti i ngā whakapaunga whakamātautau mā te 70% ki ngā kete tātaritanga kotahi

Whakahaere mōreareaNgā whakatūpato pai wawe me te tautoko whakaū HPLC-MS/MS

Ngā horopaki tono:

Te kai miraka mata i ngā teihana kohikohi

Ngā arowhai kounga o roto i ngā whare miraka

Ngā tirotiro ā-papa ture

Te Whakapūmau Kounga

Te roa o te oranga mō ngā marama 12 (2-8°C te penapena i roto i te pōuri, KAUA E WHAKATIO)

Te whai i ngā puranga: Tau rota me te rā whakaputa i runga i te takai

Otinga katoa: Hototahi ki ngā incubator me ngā tātari koura colloidal

Whakamutunga

Ka hurihia e te MilkGuard® 16-i roto-i-1 te tirotiro paturopi mā te whakapoto i ngā rerengamahi uaua ki roto i tētahi tukanga kotahi-taahiraa, 9-meneti te roa – ka whakamana i ngā umanga miraka ki te whakatinana i te tutuki i ngā whakaritenga kore-toenga.
Mātakihia te ataata whakaaturanga: