Ubushinwa Immunoaffinity inkingi ya Ochratoxin Uruganda rukora ibicuruzwa nababikora

Immunoaffinity inkingi ya Ochratoxin Kumenyekanisha Ishusho Yihariye

Immunoaffinity inkingi ya Ochratoxin Kumenya

Ibisobanuro bigufi:

Kwinbon Ochratoxin Inkingi zikoreshwa muguhuza na HPLC, LC-MS, ELISA ibikoresho byo gupima. Irashobora kuba igeragezwa ryinshi Ochratoxin A kubinyampeke nibicuruzwa byimbuto, isosi ya soya, vinegere, ibicuruzwa bya sosi, inzoga, Cocoa hamwe nikawa ikaranze, nibindi.

Ohereza imeri kuri twe Amabwiriza yo gukoresha

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro ku bicuruzwa

Injangwe no.	KH00404Z
Ibyiza	KuriOchratoxin A ikizamini
Aho yaturutse	Beijing, Ubushinwa
Izina ry'ikirango	Kwinbon
Ingano yubumwe	Ibizamini 25 kuri buri gasanduku
Icyitegererezo	Gimvura n'ibicuruzwa by'ingano, isosi ya soya, vinegere, ibicuruzwa by'isosi, inzoga, Kakao n'ikawa ikaranzen'ibindi.
Ububiko	2-30 ℃
Ubuzima bwa Shelf	Amezi 12
Gutanga	Icyumba cy'ubushyuhe

Ibikoresho & Reagents Birakenewe

Ibikoresho

Ibikoresho bitanga umusaruro

Ibikoresho

---- Homogenizer ---- Vortex ivanga

---- Icupa ry'icyitegererezo ---- Gupima silinderi: 10ml, 100ml

---- Impapuro zungurura impapuro / Centrifuge ---- Impuzandengo yisesengura (inductance: 0.01g)

---- Impamyabumenyi yarangije: 10ml ---- Injiza: 20ml

---- Amashanyarazi ya volumetric: 250ml ---- Rubber pipette

---- Micropipette: 100-1000ul ---- Glass funnel 50ml

---- Akayunguruzo ka Microfibre (Whatman, 934-AH, Φ11cm, 1.5um ruziga)

Ibikoresho bitanga umusaruro

---- Methanol (AR)

---- Acide acike (AR)

---- Sodium chloride (NACL, AR)

---- Amazi ya Deionised

Ibyiza byibicuruzwa

Nkuko bizwi na mycotoxine, Ochratoxin A (OTA) ikorwa nubwoko butandukanye bwibihumyo harimo Aspergillus ochraceus, A. karubone, A. niger na Penicillium verrucosum. OTA itera nephrotoxicity n'ibibyimba by'impyiko mu bwoko butandukanye bw'inyamaswa; icyakora, ingaruka zubuzima bwabantu ntizirangwa neza.

Kwinbon Inmmunoaffinity Inkingi nuburyo bwa gatatu, ikoresha chromatografiya yamazi yo gutandukana, kwezwa cyangwa gusesengura byihariye bya Ochratoxin A. Mubisanzwe inkingi za Kwinbon zahujwe na HPLC.

Isesengura ry’uburozi bwa HPLC ku bihumyo ni uburyo bwo kumenya uburozi bw’ibihumyo bukuze. Haba imbere na inyuma birakoreshwa. HPLC ikoreshwa mu buryo buhendutse, yoroshye kuyikoresha, kandi ifite uburozi buke bwo gushonga. Uburozi bwinshi bushongeshwa mu bice byo mu kirere hanyuma bugatandukanywa n’inkingi zitari zo mu kirere, bihaza ibyifuzo byo kubona uburozi bwinshi bw’ibihumyo mu gipimo cy’amata. Utwuma duto twa UPLC dukoreshwa buhoro buhoro, hamwe n’uduce duto twa chromatography, dushobora kugabanya igihe cyo gukora ingero, kunoza imikorere yo gutandukanya chromatography, no kugera ku buhanga bwo hejuru.

Hamwe nibisobanuro bihanitse, Kwinbon Ochratoxin Inkingi irashobora gufata molekile yintego muburyo bwiza cyane. Nanone inkingi za Kwinbon zitemba vuba, byoroshye gukora. Noneho birihuta kandi bikoreshwa cyane mubiryo no murima ya mycotoxine decetion.