Ubushinwa Immunoaffinity inkingi ya T-2 mycotoxin detection uruganda nababikora

Immunoaffinity inkingi ya T-2 mycotoxin

Ibisobanuro bigufi:

Kwinbon T-2 inkingi ya mycotoxin ikoreshwa muguhuza HPLC, LC-MS, ELISA ibikoresho byo gupima.Irashobora kuba igeragezwa ryinshi T-2 mycotoxine kubinyampeke n'ibinyampeke, isosi ya soya, vinegere, ibikomoka ku isosi, inzoga, soya, amavuta y'imboga n'imboga, kugaburira ibikoresho fatizo n'ibicuruzwa byarangiye, n'ibindi.

Ohereza imeri kuri twe Amabwiriza yo Gukoresha

Ibicuruzwa birambuye

Ibirango by'ibicuruzwa

Ibisobanuro ku bicuruzwa

Injangwe no.	KH01301Z
Imitungo	KuriT-2 mycotoxinikizamini
Aho byaturutse	Beijing, Ubushinwa
Izina ry'ikirango	Kwinbon
Ingano yubumwe	Ibizamini 25 kuri buri gasanduku
Urugero rw'ikoreshwa	Ibinyampeke n'ibinyampeke, isosi ya soya, vinegere, ibicuruzwa by'isosi, inzoga, n'ibindi.
Ububiko	2-30℃
Igihe cyo kumara	Amezi 12
Gutanga	Icyumba cy'ubushyuhe

Ibikoresho & Reagents Birakenewe

Ibikoresho

Reagents

Ibikoresho

----Homogenizer ----Vortex mixer

---- Icupa ry'icyitegererezo ---- Gupima silinderi: 10ml, 100ml

---- Impapuro zungurura impapuro / Centrifuge ---- Impuzandengo yisesengura (inductance: 0.01g)

---- Impamyabumenyi yarangije: 10ml ---- Injiza: 20ml

---- Amashanyarazi ya volumetric: 250ml ---- Rubber pipette

---- Micropipette: 100-1000ul ---- Glass funnel 50ml

---- Akayunguruzo ka Microfibre (Whatman, 934-AH, Φ11cm, 1.5um ruziga)

Reagents

---- Methanol (AR)

---- Acide acike (AR)

---- Sodium chloride (NACL, AR)

---- Amazi yakuwemo ioni

Ibyiza by'ibicuruzwa

T-2 mycotoxine ni ibisanzwe bisanzwe biboneka byumusaruro wa Fusarium spp. fungus kandi ni uburozi kubantu nandi matungo. Imiterere yubuvuzi itera ni alimentary toxic aleukia hamwe nibimenyetso byinshi bifitanye isano ningingo zitandukanye nkuruhu, umwuka, nigifu. Iraboneka muri T-2 mycotoxine ibinyampeke byanduye, ibiryo n'ibiryo.

Kwinbon Inmmunoaffinity Inkingi ni uburyo bwa gatatu, ikoresha chromatografiya y'amazi yo gutandukanya, kweza cyangwa gusesengura byihariye bya T-2 mycotoxine. Mubisanzwe inkingi za Kwinbon zahujwe na HPLC.

HPLC isesengura ryinshi ryuburozi bwa fungal nubuhanga bukuze bwo kumenya. Byombi imbere na revers ya chromatografi irakoreshwa. Guhindura icyiciro HPLC nubukungu, byoroshye gukora, kandi bifite uburozi buke. Uburozi bwinshi burashonga mubice byimikorere ya polar hanyuma bigatandukanywa ninkingi za chromatografiya zidafite inkingi, byujuje ibisabwa kugirango hamenyekane vuba uburozi bwinshi bwibihumyo mubyitegererezo byamata. UPLC ihuriweho na disiketi igenda ikoreshwa buhoro buhoro, hamwe ningutu yumuvuduko mwinshi hamwe nubunini buto hamwe nubunini bwa chromatografiya, bishobora kugabanya icyitegererezo cyo gukora, kunoza imikorere ya chromatografique, kandi bikagera no kubyumva neza.

Kubera ubuhanga buhanitse, inkingi za Kwinbon T-2 mycotoxin zishobora gufata molekile zigamije mu buryo busukuye cyane. Nanone inkingi za Kwinbon zitembera vuba kandi byoroshye gukora. Ubu zikoreshwa cyane mu buryo bwihuse mu biribwa no mu binyampeke mu gushuka mycotoxins.