Inkingi za Immunoaffinity zo mu Bushinwa zo gupima Zearalenone n'abazikora

Inkingi z'imyuga zikoreshwa mu gupima Zearalenone Ishusho yagaragaye

Inkingi z'imyuga zikoreshwa mu gusuzuma Zearalenone

Ibisobanuro bigufi:

Inkingi za Kwinbon Zearalenone zikoreshwa mu guhuza na HPLC, LC-MS, na ELISA. Bishobora gupimwa AFB1 ku binyampeke n'ibikomoka ku binyampeke, isosi ya soya, vinegere, ibikomoka ku isosi, inzoga, soya, amavuta ya rapeseed n'ibikomoka ku bimera, ibikoresho fatizo byo kugaburira n'ibikomoka ku bimera byarangiye, nibindi.

Ohereza imeri kuri twe Amabwiriza yo gukoresha

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ibipimo by'ibicuruzwa

Injangwe nimero.	KH00304Z
Imitungo	KuriZearalenoneikizamini
Aho yaturutse	Beijing, Ubushinwa
Izina ry'ikirango	Kwinbon
Ingano y'igipimo	Ibizamini 25 kuri buri gasanduku
Urugero rw'ikoreshwa	Ibinyampeke n'ibikomoka ku binyampeke, isosi ya soya, vinegere, ibikomoka ku isosi, inzoga, soya, amavuta y'ibimera n'ay'ibimera, ibikoresho fatizo byo kugaburira n'ibikomoka ku bimera, n'ibindi.
Ububiko	2-30℃
Igihe cyo kumara	Amezi 12
Gutanga	Ubushyuhe bw'icyumba

Ibikoresho n'ibikoresho bitanga umusaruro birakenewe

Ibikoresho

Ibikoresho bitanga umusaruro

Ibikoresho

----Homogenizer ----Vortex mixer

----Icupa ry'icyitegererezo ----Silinda yo gupima: 10ml, 100ml

----Impapuro ziyungurura neza/Centrifuge ----Ingano y'isesengura (inductance: 0.01g)

----Umuyoboro warangije: 10ml ----Umushongi: 20ml

----Icupa ry'ingano: 250ml ----Itara ry'umuyoboro wa rubber

----Mikoropipeti: 100-1000ul ----Umuyoboro w'ikirahure 50ml

----Imashini ziyungurura za Microfiber (Whatman, 934-AH, Φ11cm, uruziga rwa 1.5um)

Ibikoresho bitanga umusaruro

----Methanol (AR)

----aside asetiki (AR)

----Sodium chloride (NACL,AR)

---- Amazi yakuwemo ioni

Ibyiza by'ibicuruzwa

Zearalenone (ZEN), izwi kandi nka RAL na F-2 mycotoxin, ni metabolite ikomeye ya estrogenic ikorwa. Irinda ubushyuhe kandi iboneka ku isi yose mu bihingwa byinshi by'ibinyampeke, nk'ibigori, sayiri, ingano, ingano, umuceri n'amasaka.

Zearalenone ni yo burozi bw'ibanze bufatana n'udukingirizo twa estrogen, bigatera ubugumba, gukuramo inda cyangwa ibindi bibazo byo kororoka, cyane cyane mu ngurube.

Wikipedia itanga inama ku buryo bukurikira bwo gutahura;

Koromatografiya y'urwego ruciriritse,
Isuzuma ry'imyunyungugu rifitanye isano na enzyme
Umucyo w'inkingi ya immunoaffinity
Chromatography y'amazi ifite ubushobozi bwo hejuru bwo gukoresha immunoaffinity column

Kwinbon Inmmunoaffinity Columns ni uburyo bwa gatatu, bukoresha chromatography y'amazi mu gutandukanya, gutunganya cyangwa gusesengura byihariye Zearalenone. Ubusanzwe inkingi za Kwinbon zihuzwa na HPLC.

Isesengura ry’uburozi bwa HPLC ku bihumyo ni uburyo bwo kumenya uburozi bw’ibihumyo bukuze. Haba imbere na inyuma birakoreshwa. HPLC ikoreshwa mu buryo buhendutse, yoroshye kuyikoresha, kandi ifite uburozi buke bwo gushonga. Uburozi bwinshi bushongeshwa mu bice byo mu kirere hanyuma bugatandukanywa n’inkingi zitari zo mu kirere, bihaza ibyifuzo byo kubona uburozi bwinshi bw’ibihumyo mu gipimo cy’amata. Utwuma duto twa UPLC turimo gukoreshwa buhoro buhoro, hamwe n’uduce duto twa chromatography, dushobora kugabanya igihe cyo gukora ingero, kunoza imikorere yo gutandukanya chromatography, no kugera ku buhanga bwo hejuru.

Kubera ubuhanga buhanitse, inkingi za Kwinbon Zearalenone zishobora gufata molekile zigamije mu buryo busukuye cyane. Nanone inkingi za Kwinbon zitembera vuba kandi byoroshye gukora. Ubu zikoreshwa cyane mu buryo bwihuse mu biribwa no mu binyampeke mu gushuka uburozi bwa mycotoxins.