ibicuruzwa

Nitrofurans zishyirwa mu mubiri vuba cyane, kandi metabolites zazo zihujwe n'uturemangingo zishobora kubaho igihe kirekire, bityo isesengura ry'ibisigazwa by'iyi miti rizaterwa no kumenya metabolites zayo, harimo metabolite ya furazolidone (AOZ), metabolite ya furaltadone (AMOZ), metabolite ya nitrofurantoin (AHD) na metabolite ya nitrofurazone (SEM).

Ibikoresho bya Kwinbon Competitive Enzyme Immunoassay, bizwi kandi nka Elisa kits, ni ikoranabuhanga rikoreshwa mu gusuzuma indwara hashingiwe ku ihame rya Enzyme-Linked Immunosorbent Assay (ELISA). Ibyiza byabyo bigaragarira ahanini muri ibi bikurikira:

 (1) Kwihuta: Ubusanzwe laboratwari zikoresha LC-MS na LC-MS/MS kugira ngo zimenye metabolite ya nitrofurazone. Ariko ikizamini cya Kwinbon ELISA, aho antibody yihariye ya SEM derivative iba nziza kurushaho, yumvikana, kandi yoroshye kuyikoresha. Igihe cyo gupima iyi kit ni isaha 1.5 gusa, bikaba ari ingirakamaro cyane kugira ngo haboneke ibisubizo. Ibi ni ingenzi mu gusuzuma vuba no kugabanya imbaraga z'akazi.

(2) Ubuziranenge: Bitewe n’uburyo bworoshye bwo kumenya no gusobanukirwa ibintu bya Kwinbon SEM Elisa, ibisubizo ni ukuri cyane kandi nta makosa menshi afite. Ibi bituma ikoreshwa cyane muri laboratwari z’ubuvuzi n’ibigo by’ubushakashatsi kugira ngo ifashe ubworozi bw’amafi n’abohereza ibicuruzwa byo mu mazi mu gusuzuma no gukurikirana ibisigazwa by’imiti ya SEM mu bikomoka ku mazi.

(3) Ubuhanga bwo hejuru: Kwinbon SEM Elisa kit ifite ubushobozi bwo gupima neza kandi ishobora gupimwa ukoresheje antibody runaka. Uburyo SEM na metabolite yayo bihura na 100%. Uburyo Corss ikora bugaragaza 0.1% munsi ya AOZ, AMOZ, AHD, CAP na metabolite zayo, bifasha kwirinda kwibeshya no kudakora isuzuma.