Iikholamu ze-Immunoaffinity zaseTshayina zefektri yokuchonga i-Aflatoxin B1 kunye nabavelisi

Iikholamu ze-Immunoaffinity zokufumanisa i-Aflatoxin B1

Inkcazo emfutshane:

Iikholamu zeKwinbon Aflatoxin B1 zisetyenziswa ngokudityaniswa nekhithi yovavanyo lweHPLC, LC-MS, ELISA. Ingavavanywa ngobuninzi i-AFB1 kwiinkozo, amandongomane kunye neemveliso zawo, iioyile zemifuno kunye namafutha, iimveliso zamandongomane, isosi yesoya, iviniga, amayeza aseTshayina, iziqholo kunye neti.

Thumela i-imeyile kuthi Imiyalelo Yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha zemveliso

Inombolo yekati.	KH01104Z
Iipropati	Uvavanyo lwe-Aflatoxin B1
Indawo yemvelaphi	EBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Uvavanyo lwe-25 kwibhokisi nganye
Isicelo seSampuli	Iinkozo, amandongomane kunye neemveliso zawo, iioyile zemifuno kunye namafutha, iimveliso zamandongomane, isosi yesoya, iviniga, amayeza aseTshayina, iziqholo kunye neti
Indawo yokugcina izinto	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ziyafuneka

Izixhobo

Iiarhente

Izixhobo

----Homogenizer -----Umxube weVortex

----Ibhotile yesampulu ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lesihluzo elisemgangathweni/iCentrifuge ----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Ipayipi ephumeleleyo: 10ml ----Injector: 20ml

----Iflaski yevolumu: 250ml ----Ibhalbhu yepayipi yerabha

----I-Micropipette: 100-1000ul ----I-funnel yeglasi 50ml

----Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, isangqa se-1.5um)

Iiarhente

----iMethanol (AR)

----I-asidi ye-acetic (AR)

----Isodiyam chloride (NACL,AR)

-----Amanzi anyibilikisiweyo

Iingenelo zemveliso

I-Aflatoxin B1 sisingcolisi esiqhelekileyo kwiintlobo ngeentlobo zokutya kuquka amandongomane, umgubo wekotoni, umbona, kunye nezinye iinkozo; kunye nokutya kwezilwanyana. I-Aflatoxin B1 ithathwa njengeyona aflatoxin inetyhefu kwaye ichaphazeleka kakhulu kwi-hepatocellular carcinoma (HCC) ebantwini.

IWikipedia icebisa ezi ndlela zilandelayo zokufumanisa;

I-chromatography yomaleko obhityileyo
Uvavanyo lwe-immunosorbent oludityaniswe ne-enzyme
Ukukhanya kwekholamu ye-Immunoaffinity
I-chromatography yolwelo olusebenza kakuhle kwikholamu ye-Immunoaffinity

I-Kwinbon Inmmunoaffinity Columns yindlela yesithathu, isebenzisa i-liquid chromatography ukwahlula, ukucoca okanye ukuhlalutya i-Aflatoxin B1. Ngokwesiqhelo iikholamu ze-Kwinbon zidityaniswe ne-HPLC.

Uhlalutyo lobungakanani lwe-HPLC lweetyhefu zefungus yindlela yokubona evuthiweyo. Zombini i-chromatography yesigaba sangaphambili nesasemva ziyasebenza. I-HPLC yesigaba sangasemva ayibizi kakhulu, kulula ukuyisebenzisa, kwaye inetyhefu encinci ye-solvent. Uninzi lweetyhefu luyanyibilika kwizigaba ezihambayo ze-polar kwaye emva koko zahlulwe ziikholamu ze-chromatography ezingezizo ze-polar, zihlangabezana neemfuno zokufunyanwa ngokukhawuleza kweetyhefu ezininzi zefungus kwisampulu yobisi. Izixhobo zokubona ezidibeneyo ze-UPLC ziyasetyenziswa kancinci kancinci, kunye neemodyuli zoxinzelelo oluphezulu kunye neekholamu ze-chromatography ezinobukhulu obuncinci kunye nobukhulu be-particle, ezinokunciphisa ixesha lokusebenza kwesampulu, ziphucule ukusebenza kakuhle kokwahlulwa kwe-chromatographic, kwaye zifezekise uvakalelo oluphezulu.

Ngobuchule obuphezulu, iikholamu ze-Kwinbon Aflatoxin B1 zinokubamba iimolekyuli ekujoliswe kuzo zikwimeko ecocekileyo kakhulu. Kwakhona iikholamu ze-Kwinbon zihamba ngokukhawuleza, kulula ukuzisebenzisa. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwindawo yokutya nakwingqolowa ukukhohlisa ii-mycotoxins.