Iintsika ze-China Immunoaffinity ze-Aflatoxin B1 zokubona umzi-mveliso kunye nabavelisi

Iintsika ze-Immunoaffinity zokufunyaniswa kwe-Aflatoxin B1 Umfanekiso ofakiweyo

Iikholamu ze-Immunoaffinity zokufunyanwa kwe-Aflatoxin B1

Inkcazelo emfutshane:

Iikholamu zeKwinbon Aflatoxin B1 zisetyenziswa ngokudibanisa neHPLC, LC-MS, ELISA test kit. Ingaluvavanyo lobungakanani be-AFB1 yeesiriyeli, amandongomane kunye neemveliso zawo, ioyile yemifuno kunye namafutha, iimveliso zendongomane, isosi yesoya, iviniga, amayeza aseTshayina, izinongo kunye neti.

Thumela i-imeyile kuthi Imikhomba-ndlela yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iimpawu zemveliso

Inombolo yekati.	KH01104Z
Iipropati	Kuvavanyo lwe-Aflatoxin B1
Indawo yemvelaphi	eBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Iimvavanyo ezingama-25 kwibhokisi nganye
Isampulu yosetyenziso	Iisiriyeli, amandongomane kunye neemveliso zawo, ioyile yemifuno kunye namafutha, iimveliso zenut, isosi yesoya, iviniga, amayeza aseTshayina, iziqholo kunye neti.
Ugcino	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ezifunekayo

Izixhobo

Iireagents

Izixhobo

----Homogenizer ----Umxube weVortex

----Ibhotile yesampuli ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lesihluzo elisemgangathweni/iCentrifuge ----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Uthweswe isidanga: 10ml ----Injector: 20ml

----Iflaski yeVolumetric: 250ml ----Ibhalbhu yerabha yepayipi yerabha

-----Micropipette: 100-1000ul ---- Ifaneli yeglasi 50ml

---- Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, 1.5um isangqa)

Iireagents

----Methanol (AR)

----Acetic acid (AR)

-----I-sodium chloride (NACL,AR)

---- Amanzi agayiweyo

Iinzuzo zemveliso

I-Aflatoxin B1 yingcoliseko eqhelekileyo kwiindidi zokutya okuquka amandongomane, i-cottonseed meal, umbona, kunye nezinye iinkozo; ngokunjalo nokutya kwezilwanyana. I-Aflatoxin B1 ithathwa njengeyona aflatoxin inetyhefu kwaye ibandakanyeka kakhulu kwi-hepatocellular carcinoma (HCC) ebantwini.

I-Wikipedia icebisa ezi ndlela zilandelayo zokubona;

Umaleko omncinci wechromatography
Uvavanyo lwe-immunosorbent olunxulumene ne-enzyme
I-Immunoaffinity column fluorescence
Ikholamu ye-Immunoaffinity yokusebenza okuphezulu kwechromatography yolwelo

Kwinbon Inmmunoaffinity Columns yeyendlela yesithathu, isebenzisa ulwelo lwechromatography ukwahlula, ukucocwa okanye uhlalutyo oluthile lwe-Aflatoxin B1. Ngesiqhelo iikholamu zeKwinbon zidityaniswe neHPLC.

Uhlalutyo lobungakanani be-HPLC yetyhefu yokungunda bubuchule bokubona obuqolileyo. Zombini i-chromatography yesigaba sangaphambili kunye nesigaba esingasemva siyasebenza. Isigaba sokubuyela umva i-HPLC inoqoqosho, kulula ukuyisebenzisa, kwaye inetyhefu ye-solvent ephantsi. Uninzi lwee-toxins ziyanyibilika kwizigaba ezihamba ngepolar kwaye emva koko zahlulwe ziikholamu zechromatography ezingeyopolar, zihlangabezana neemfuno zokubhaqwa ngokukhawuleza kweetoxin ezininzi zefungal kwisampulu yobisi. I-UPLC i-detectors edibeneyo isetyenziswa ngokuthe ngcembe, kunye neemodyuli eziphezulu zoxinzelelo kunye nobukhulu obuncinci kunye neekholamu ze-chromatography ze-particle size inokunciphisa ixesha lokuhamba kwesampuli, ukuphucula ukusebenza kakuhle kokwahlula kwechromatographic, kunye nokufezekisa uvakalelo oluphezulu.

Ngobuchule obuphezulu, iikholamu ze-Kwinbon Aflatoxin B1 zinokubamba iimolekyuli ekujoliswe kuzo zikwimeko ecocekileyo kakhulu. Kwakhona iikholamu ze-Kwinbon zihamba ngokukhawuleza, kulula ukuzisebenzisa. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwindawo yokutya nakwingqolowa ukukhohlisa ii-mycotoxins.