Iikholamu ze-Immunoaffinity zaseTshayina ze-Aflatoxin Total mveliso kunye nabavelisi

Iikholamu ze-Immunoaffinity ze-Aflatoxin Umfanekiso opheleleyo oFakiweyo

Iikholamu ze-Immunoaffinity ze-Aflatoxin Total

Inkcazo emfutshane:

Iikholamu ze-AFT zisetyenziswa ngokudityaniswa nekhithi yovavanyo ye-HPLC, i-LC-MS, ne-ELISA.

Ingavavanywa ngobuninzi i-AFB1, AFB2, AFG1, AFG2. Ifanelekile kwiinkozo, ukutya, amayeza aseTshayina, njl.njl. kwaye iphucula ubunyulu beesampuli.

Thumela i-imeyile kuthi Imiyalelo Yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha zemveliso

Inombolo yekati.	KH01102Z
Iipropati	Uvavanyo lwe-Aflatoxin Total
Indawo yemvelaphi	EBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Uvavanyo lwe-25 kwibhokisi nganye
Isicelo seSampuli	Ukutya, Ingqolowa, Iinkozo kunye neziqholo
Indawo yokugcina izinto	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ziyafuneka

Izixhobo

Iiarhente

Izixhobo

----Homogenizer -----Umxube weVortex

----Ibhotile yesampulu ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lesihluzo elisemgangathweni/iCentrifuge ----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Ipayipi ephumeleleyo: 10ml ----Injector: 20ml

----Iflaski yevolumu: 250ml ----Ibhalbhu yepayipi yerabha

----I-Micropipette: 100-1000ul ----I-funnel yeglasi 50ml

----Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, isangqa se-1.5um)

Iiarhente

----iMethanol (AR)

----I-asidi ye-acetic (AR)

----Isodiyam chloride (NACL,AR)

-----Amanzi anyibilikisiweyo

Iingenelo zemveliso

Iikholamu zeKwinbon Inmmunoaffinity zisebenzisa i-chromatography yolwelo ukwahlula, ukucoca okanye uhlalutyo oluthile lwe-Aflatoxin Total. Ngokwesiqhelo iikholamu zeKwinbon zidityaniswe ne-HPLC.
I-antibody ye-monoclonal echasene ne-Aflatoxin Total idibene ne-coagulating media kwikholamu. Ii-Mycotoxins kwisampuli ziyakhutshwa, zihluzwe kwaye zixutywe. Yenza isisombululo sokukhupha isampuli sidlule kwikholamu ye-Aflatoxin Total. Intsalela ye-Aflatoxin (B1, B2, G1, G2) idityaniswe ne-antibody ngokwahlukeneyo kwikholamu, isisombululo sokuhlamba sisusa ukungcola okungadibaniswanga. Okokugqibela, kusetyenziswa i-methyl alcohol ukususa i-Aflatoxin B1, i-Aflatoxin B2, i-Aflatoxin G1, i-Aflatoxin G2.
Ngobuchule obuphezulu, iikholamu ze-Kwinbon AFT zinokubamba iimolekyuli ekujoliswe kuzo zikwimeko ecocekileyo kakhulu. Kwakhona iikholamu ze-Kwinbon zihamba ngokukhawuleza, kulula ukuzisebenzisa. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwintsimi yokutya kunye nengqolowa ukukhohlisa ii-mycotoxins.