Iikholamu ze-Immunoaffinity zaseTshayina zefektri yokuchonga iFumonisin kunye nabavelisi

Iikholamu ze-Immunoaffinity zokufumanisa i-Fumonisin

Inkcazo emfutshane:

Iikholamu zeKwinbon Fumonisin zisetyenziswa ngokudityaniswa nekhithi yovavanyo yeHPLC, LC-MS, ELISA. Ifanelekile kwiinkozo, ukutya, amayeza aseTshayina, njl.njl. kwaye iphucula ubunyulu beesampuli.

Thumela i-imeyile kuthi Imiyalelo Yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha zemveliso

Inombolo yekati.	KH01001Z
Iipropati	NgokubaI-Fumonisinuvavanyo
Indawo yemvelaphi	EBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Uvavanyo lwe-25 kwibhokisi nganye
Isicelo seSampuli	Ukutya, Ingqolowa, Iinkozo kunye neziqholo
Indawo yokugcina izinto	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ziyafuneka

Izixhobo

Iiarhente

Izixhobo

----Homogenizer -----Umxube weVortex

----Ibhotile yesampulu ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lesihluzo elisemgangathweni/iCentrifuge ----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Ipayipi ephumeleleyo: 10ml ----Injector: 20ml

----Iflaski yevolumu: 250ml ----Ibhalbhu yepayipi yerabha

----I-Micropipette: 100-1000ul ----I-funnel yeglasi 50ml

----Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, isangqa se-1.5um)

Iiarhente

----iMethanol (AR)

----I-asidi ye-acetic (AR)

----Isodiyam chloride (NACL,AR)

-----Amanzi anyibilikisiweyo

Iingenelo zemveliso

I-Fomonisin (FB) luhlobo lwee-metabolites ezinyibilikayo emanzini eziveliswa yi-fungi ebangela ubuthi. Ingcolisa kakhulu iinkozo kunye neemveliso zazo, kwaye inetyhefu ebukhali kwaye inokubangela umhlaza kwimfuyo ethile, kwaye ibe yenye indawo ephambili yophando emva kwe-aflatoxin. Ingabangela umonakalo omkhulu ebantwini nakwizilwanyana kuba le nto ifumaneka kakhulu embona nakwezinye iimveliso zezolimo. Kutshanje i-fumonisin ibe yenye yezona ndawo zingcolisayo kwihlabathi liphela. Uphando malunga nokuthintela nokulawula i-fumonisin lube yindawo ephambili yophando kwihlabathi liphela.

IWikipedia icebisa ezi ndlela zilandelayo zokufumanisa;

I-chromatography yomaleko obhityileyo,
Uvavanyo lwe-immunosorbent oludityaniswe ne-enzyme
Ukukhanya kwekholamu ye-Immunoaffinity
I-chromatography yolwelo olusebenza kakuhle kwikholamu ye-Immunoaffinity

I-Kwinbon Inmmunoaffinity Columns yindlela yesithathu, isebenzisa i-liquid chromatography yokwahlula, ukucoca okanye uhlalutyo oluthile lwe-Fumonisin. Ngokwesiqhelo iikholamu ze-Kwinbon zidityaniswe ne-HPLC.

Uhlalutyo lobungakanani lwe-HPLC lweetyhefu zefungus yindlela yokubona evuthiweyo. Zombini i-chromatography yesigaba sangaphambili nesasemva ziyasebenza. I-HPLC yesigaba sangasemva ayibizi kakhulu, kulula ukuyisebenzisa, kwaye inetyhefu encinci ye-solvent. Uninzi lweetyhefu luyanyibilika kwizigaba ezihambayo ze-polar kwaye emva koko zahlulwe ziikholamu ze-chromatography ezingezizo ze-polar, zihlangabezana neemfuno zokufunyanwa ngokukhawuleza kweetyhefu ezininzi zefungus kwisampulu yobisi. Izixhobo zokubona ezidibeneyo ze-UPLC ziyasetyenziswa kancinci kancinci, kunye neemodyuli zoxinzelelo oluphezulu kunye neekholamu ze-chromatography ezinobukhulu obuncinci kunye nobukhulu be-particle, ezinokunciphisa ixesha lokusebenza kwesampulu, ziphucule ukusebenza kakuhle kokwahlulwa kwe-chromatographic, kwaye zifezekise uvakalelo oluphezulu.

Ngobuchule obuphezulu, iikholamu zeKwinbon Fumonisin zinokubamba iimolekyuli ekujoliswe kuzo zikwimeko ecocekileyo kakhulu. Kwakhona iikholamu zeKwinbon zihamba ngokukhawuleza, kulula ukuzisebenzisa. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwindawo yokutya nakwingqolowa ukukhohlisa ii-mycotoxins.