Iikholamu ze-Immunoaffinity zaseTshayina ze-Ochratoxin A zokuchonga kunye nabavelisi

Iikholamu ze-Immunoaffinity ze-Ochratoxin Ukufunyanwa komfanekiso obonisiweyo

Iikholamu ze-Immunoaffinity zokufumanisa i-Ochratoxin A

Inkcazo emfutshane:

Iikholamu zeKwinbon Ochratoxin A zisetyenziswa ngokudityaniswa nekhithi yovavanyo yeHPLC, LC-MS, ELISA. Ingavavanywa ngobuninzi iOchratoxin A kwiinkozo kunye neemveliso zeenkozo, isosi yesoya, iviniga, iimveliso zesosi, utywala, iCocoa kunye nekofu eyosiweyo, njl.njl.

Thumela i-imeyile kuthi Imiyalelo Yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha zemveliso

Inombolo yekati.	KH00404Z
Iipropati	NgokubaI-Ochratoxin A uvavanyo
Indawo yemvelaphi	EBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Uvavanyo lwe-25 kwibhokisi nganye
Isicelo seSampuli	Giimveliso zemvula kunye neenkozo, isosi yesoya, iviniga, iimveliso zesosi, utywala, iCocoa kunye nekofu eyosiweyo, njl. njl.
Indawo yokugcina izinto	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ziyafuneka

Izixhobo

Iiarhente

Izixhobo

----Homogenizer -----Umxube weVortex

----Ibhotile yesampulu ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lesihluzo elisemgangathweni/iCentrifuge ----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Ipayipi ephumeleleyo: 10ml ----Injector: 20ml

----Iflaski yevolumu: 250ml ----Ibhalbhu yepayipi yerabha

----I-Micropipette: 100-1000ul ----I-funnel yeglasi 50ml

----Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, isangqa se-1.5um)

Iiarhente

----iMethanol (AR)

----I-asidi ye-acetic (AR)

----Isodiyam chloride (NACL,AR)

-----Amanzi anyibilikisiweyo

Iingenelo zemveliso

Njengoko yaziwa ngokuba yi-mycotoxin, i-Ochratoxin A (OTA) eveliswa ziintlobo ezahlukeneyo zefungus kuquka i-Aspergillus ochraceus, i-A. carbonarius, i-A. niger kunye ne-Penicillium verrucosum. I-OTA ibangela ubuthi kunye neethumba zezintso kwiintlobo ngeentlobo zezilwanyana; nangona kunjalo, iziphumo zempilo yabantu azibonakali kakuhle.

I-Kwinbon Inmmunoaffinity Columns yindlela yesithathu, isebenzisa i-chromatography yolwelo ukwahlula, ukucoca okanye uhlalutyo oluthile lwe-Ochratoxin A. Ngokwesiqhelo iikholamu ze-Kwinbon zidityaniswe ne-HPLC.

Uhlalutyo lobungakanani lwe-HPLC lweetyhefu zefungus yindlela yokubona evuthiweyo. Zombini i-chromatography yesigaba sangaphambili nesasemva ziyasebenza. I-HPLC yesigaba sangasemva ayibizi kakhulu, kulula ukuyisebenzisa, kwaye inetyhefu encinci ye-solvent. Uninzi lweetyhefu luyanyibilika kwizigaba ezihambayo ze-polar kwaye emva koko zahlulwe ziikholamu ze-chromatography ezingezizo ze-polar, zihlangabezana neemfuno zokufunyanwa ngokukhawuleza kweetyhefu ezininzi zefungus kwisampulu yobisi. Izixhobo zokubona ezidibeneyo ze-UPLC ziyasetyenziswa kancinci kancinci, kunye neemodyuli zoxinzelelo oluphezulu kunye neekholamu ze-chromatography ezinobukhulu obuncinci kunye nobukhulu be-particle, ezinokunciphisa ixesha lokusebenza kwesampulu, ziphucule ukusebenza kakuhle kokwahlulwa kwe-chromatographic, kwaye zifezekise uvakalelo oluphezulu.

Ngobuchule obuphezulu, iikholamu zeKwinbon Ochratoxin A zinokubamba iimolekyuli ekujoliswe kuzo zikwimeko ecocekileyo kakhulu. Kwakhona iikholamu zeKwinbon zihamba ngokukhawuleza, kulula ukuzisebenzisa. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwindawo yokutya nakwingqolowa ukukhohlisa ii-mycotoxins.