Iikholamu ze-Immunoaffinity zaseTshayina zeefektri zokuchonga iVomitoxin kunye nabavelisi

Iikholamu ze-Immunoaffinity zokufumanisa iVomitoxin

Inkcazo emfutshane:

Iikholamu zeKwinbon Vomitoxin zisetyenziswa ngokudityaniswa nekhithi yovavanyo yeHPLC, LC-MS, ELISA.Kungaba luvavanyo lobungakanani lwe-AFB1 lweenkozo, amandongomane kunye neemveliso zawo, iioyile zemifuno kunye namafutha, iimveliso zamandongomane, isosi yesoya, iviniga, amayeza aseTshayina, iziqholo kunye neti.

Thumela i-imeyile kuthi Imiyalelo Yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha zemveliso

Inombolo yekati.	KH00203Z
Iipropati	Uvavanyo lweVomitoxin
Indawo yemvelaphi	EBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Uvavanyo lwe-25 kwibhokisi nganye
Isicelo seSampuli	Iinkozo, amandongomane kunye neemveliso zawo, iioyile zemifuno kunye namafutha, iimveliso zamandongomane, isosi yesoya, iviniga, amayeza aseTshayina, iziqholo kunye neti
Indawo yokugcina izinto	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ziyafuneka

Izixhobo

Iiarhente

Izixhobo

----Homogenizer -----Umxube weVortex

----Ibhotile yesampulu ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lesihluzo elisemgangathweni/iCentrifuge ----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Ipayipi ephumeleleyo: 10ml ----Injector: 20ml

----Iflaski yevolumu: 250ml ----Ibhalbhu yepayipi yerabha

----I-Micropipette: 100-1000ul ----I-funnel yeglasi 50ml

----Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, isangqa se-1.5um)

Iiarhente

----iMethanol (AR)

----I-asidi ye-acetic (AR)

----Isodiyam chloride (NACL,AR)

-----Amanzi anyibilikisiweyo

Iingenelo zemveliso

I-Vomitoxin, ekwaziwa ngokuba yi-deoxynivalenol (DON), yi-epoxy-sesquiterpenoid. Ifumaneka kakhulu kwiinkozo ezifana nengqolowa, irhasi, i-oats, i-rye, kunye nombona, kwaye ayifumaneki rhoqo kwirayisi, i-sorghum, kunye ne-triticale, nto leyo ebangela ukugabha kunye ne-anorexia kwizilwanyana kwaye inokukhokelela kwisicaphucaphu kunye nokuhlanza ebantwini.

IWikipedia icebisa ezi ndlela zilandelayo zokufumanisa;

I-chromatography yomaleko obhityileyo,
Uvavanyo lwe-immunosorbent oludityaniswe ne-enzyme
Ukukhanya kwekholamu ye-Immunoaffinity
I-chromatography yolwelo olusebenza kakuhle kwikholamu ye-Immunoaffinity

I-Kwinbon Inmmunoaffinity Columns yindlela yesithathu, isebenzisa i-liquid chromatography yokwahlula, ukucoca okanye uhlalutyo oluthile lwe-Vomitoxin. Ngokwesiqhelo iikholamu ze-Kwinbon zidityaniswe ne-HPLC.

Uhlalutyo lobungakanani lwe-HPLC lweetyhefu zefungus yindlela yokubona evuthiweyo. Zombini i-chromatography yesigaba sangaphambili nesasemva ziyasebenza. I-HPLC yesigaba sangasemva ayibizi kakhulu, kulula ukuyisebenzisa, kwaye inetyhefu encinci ye-solvent. Uninzi lweetyhefu luyanyibilika kwizigaba ezihambayo ze-polar kwaye emva koko zahlulwe ziikholamu ze-chromatography ezingezizo ze-polar, zihlangabezana neemfuno zokufunyanwa ngokukhawuleza kweetyhefu ezininzi zefungus kwisampulu yobisi. Izixhobo zokubona ezidibeneyo ze-UPLC ziyasetyenziswa kancinci kancinci, kunye neemodyuli zoxinzelelo oluphezulu kunye neekholamu ze-chromatography ezinobukhulu obuncinci kunye nobukhulu be-particle, ezinokunciphisa ixesha lokusebenza kwesampulu, ziphucule ukusebenza kakuhle kokwahlulwa kwe-chromatographic, kwaye zifezekise uvakalelo oluphezulu.

Ngobuchule obuphezulu, iikholamu zeKwinbon Vomitoxin zinokubamba iimolekyuli ekujoliswe kuzo zikwimeko ecocekileyo kakhulu. Kwakhona iikholamu zeKwinbon zihamba ngokukhawuleza, kulula ukuzisebenzisa. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwindawo yokutya nakwingqolowa ukukhohlisa ii-mycotoxins.