Iikholamu ze-Immunoaffinity zaseTshayina zeefektri zokuchonga zeZearalenone kunye nabavelisi

Iikholamu ze-Immunoaffinity zokufumanisa iZearalenone

Inkcazo emfutshane:

Iikholamu zeKwinbon Zearalenone zisetyenziswa ngokudityaniswa nekhithi yovavanyo yeHPLC, LC-MS, ELISA. Ingavavanywa ngobuninzi i-AFB1 yeenkozo kunye neemveliso zengqolowa, isosi yesoya, iviniga, iimveliso zesosi, utywala, iisoya, i-rapeseed kunye neeoyile zemifuno, izinto zokutya ezikrwada kunye neemveliso ezigqityiweyo, njl.njl.

Thumela i-imeyile kuthi Imiyalelo Yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha zemveliso

Inombolo yekati.	KH00304Z
Iipropati	NgokubaI-Zearalenoneuvavanyo
Indawo yemvelaphi	EBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Uvavanyo lwe-25 kwibhokisi nganye
Isicelo seSampuli	Iinkozo kunye neemveliso zengqolowa, isosi yesoya, iviniga, iimveliso zesosi, utywala, iisoya, iioyile zerapeseed kunye nemifuno, izinto zokutya kunye neemveliso ezigqityiweyo, njl.
Indawo yokugcina izinto	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ziyafuneka

Izixhobo

Iiarhente

Izixhobo

----Homogenizer -----Umxube weVortex

----Ibhotile yesampulu ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lesihluzo elisemgangathweni/iCentrifuge ----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Ipayipi ephumeleleyo: 10ml ----Injector: 20ml

----Iflaski yevolumu: 250ml ----Ibhalbhu yepayipi yerabha

----I-Micropipette: 100-1000ul ----I-funnel yeglasi 50ml

----Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, isangqa se-1.5um)

Iiarhente

----iMethanol (AR)

----I-asidi ye-acetic (AR)

----Isodiyam chloride (NACL,AR)

-----Amanzi anyibilikisiweyo

Iingenelo zemveliso

I-Zearalenone (ZEN), ekwaziwa ngokuba yi-RAL kunye ne-F-2 mycotoxin, yi-metabolite enamandla ye-estrogenic eveliswayo. Ihlala ishushu kwaye ifumaneka kwihlabathi liphela kwizityalo ezininzi zokutya okuziinkozo, ezifana nombona, irhasi, i-oats, ingqolowa, irayisi kunye ne-sorghum.

I-Zearalenone yeyona nto iphambili ebopha ii-estrogen receptors, nto leyo ebangela ukungazali, ukukhupha isisu okanye ezinye iingxaki zokuzala, ingakumbi kwiihagu.

IWikipedia icebisa ezi ndlela zilandelayo zokufumanisa;

I-chromatography yomaleko obhityileyo,
Uvavanyo lwe-immunosorbent oludityaniswe ne-enzyme
Ukukhanya kwekholamu ye-Immunoaffinity
I-chromatography yolwelo olusebenza kakuhle kwikholamu ye-Immunoaffinity

I-Kwinbon Inmmunoaffinity Columns yindlela yesithathu, isebenzisa i-liquid chromatography yokwahlula, ukucoca okanye uhlalutyo oluthile lweZearalenone. Ngokwesiqhelo iikholamu ze-Kwinbon zidityaniswe ne-HPLC.

Uhlalutyo lobungakanani lwe-HPLC lweetyhefu zefungus yindlela yokubona evuthiweyo. Zombini i-chromatography yesigaba sangaphambili nesasemva ziyasebenza. I-HPLC yesigaba sangasemva ayibizi kakhulu, kulula ukuyisebenzisa, kwaye inetyhefu encinci ye-solvent. Uninzi lweetyhefu luyanyibilika kwizigaba ezihambayo ze-polar kwaye emva koko zahlulwe ziikholamu ze-chromatography ezingezizo ze-polar, zihlangabezana neemfuno zokufunyanwa ngokukhawuleza kweetyhefu ezininzi zefungus kwisampulu yobisi. Izixhobo zokubona ezidibeneyo ze-UPLC ziyasetyenziswa kancinci kancinci, kunye neemodyuli zoxinzelelo oluphezulu kunye neekholamu ze-chromatography ezinobukhulu obuncinci kunye nobukhulu be-particle, ezinokunciphisa ixesha lokusebenza kwesampulu, ziphucule ukusebenza kakuhle kokwahlulwa kwe-chromatographic, kwaye zifezekise uvakalelo oluphezulu.

Ngobuchule obuphezulu, iikholamu zeKwinbon Zearalenone zinokubamba iimolekyuli ekujoliswe kuzo zikwimeko ecocekileyo kakhulu. Kwakhona iikholamu zeKwinbon zihamba ngokukhawuleza, kulula ukuzisebenzisa. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwindawo yokutya nakwingqolowa ukukhohlisa ii-mycotoxins.