iindaba

I-MilkGuard®16-kwi-1Ikhithi yoVavanyo oluKhawulezayoIqalisiwe: Isikrini 16I-antibioticIiklasi zobisi olungavuthwanga kwimizuzu eli-9

Iingenelo ezingundoqo

Uvavanyo Olupheleleyo Lweziphumo Eziphezulu

Kwangaxeshanye ibona amaqela ama-4 ee-antibiotic kwiintsalela zamayeza ezili-16:
• IiSulfonamides (SABT)
• IiQuinolones (TEQL)
• Ii-Aminoglycosides (SNKG)
• IiMacrolides (SMCF)

Izixhobo eziphambili zokuchonga:

I-Sulfadiazine (8 μg/L), iSulfadimidine (15-20 μg/L)

I-Flofenicol (0.15 μg/L, EU MRL ≤100 μg/L)

I-Monensin (5 μg/L), uColistin (10 μg/L)

Ihambelana nemida yemithetho ye-China GB 31650 kunye ne-EU EC/37/2010

Itekhnoloji yokufunda kabini

Ukutolikwa okubonakalayoThelekisa umgca wovavanyo (umgca-T) kunye nomgca wolawulo (umgca-C) ukuze ufumane umnxeba ongalunganga/olungileyo okhawulezileyo

Ukulinganiswa kwezixhobo: I-Colloidal gold analyzer izenzekela ingxelo yedatha ngokuqinisekiswa kwe-QR code batch

Ukuhamba komsebenzi okucwangcisiweyo

Inkqubo iyonke yemizuzu eli-9: Isampuli yemizuzu emi-3 yokufunxa + i-chromatography yemizuzu emi-6

Ikhadi elidibeneyo elinemicu emi-4: Ukufunyanwa okufanayo kwinqanaba elinye

Isampuli encinci: 200 μL ubisi oluluhlaza, akukho nyango lufunekayo ngaphambi kokuba lusetyenziswe

Iprotocol esemgangathweni

1. UKULUNGISELELA UMZEKELO:

- Linganisa ubisi nobushushu begumbi (bufana kakhulu)

- Fudumeza i-incubator yesinyithi ukuya kuma-45°C (Icetyiswayo: Kwinbon Mini-T4)

2. INKQUBO YOVAVANYO:

- Yongeza ubisi oluyi-200μL kwi-microwell, xuba ngokufaka imibhobho izihlandlo ezi-5

- Faka ikhadi lovavanyo (ipadi yokufunxa intywiliswe ngokupheleleyo)

3. UKUTOLIKWA (NGAPHAKATHI KWEMIZUZU EMI-2):

- IMBONAKALO:

Imbi: Ubunzulu bomgca we-T ≥ umgca we-C (intsalela < LOD)

Ilungile: Umgca-T < Umgca-C okanye ongabonakaliyo (intsalela ≥ LOD)

- ISIXHOBO SOKUSEBENZA:

Faka ikhadi kwi-analyzer → Skena ikhowudi ye-QR → Ingxelo yobungakanani ezenzekelayo

Ixabiso leShishini

Ukusebenza kakuhle kweendleko: Inciphisa iindleko zovavanyo nge-70% xa kuthelekiswa neekhithi zohlalutyo olunye

Ulawulo lomngcipheko: Izilumkiso zakwangoko ezilungileyo ngenkxaso yokuqinisekisa ye-HPLC-MS/MS

Iimeko zesicelo:

Ubisi oluvutshiweyo oluthathwa kwiindawo zokubutha

Ukuhlolwa komgangatho wemveliso kwizityalo zobisi

Uhlolo lwentsimi olulawulwayo

Ukuqinisekisa umgangatho

Ixesha lokugcinwa kweshelufu leenyanga ezili-12 (2-8°C kwindawo yokugcina emnyama, SUKUYIQANDISA)

Ukulandeleka kweqela: Inombolo yelothi kunye nomhla wokuveliswa kwiphakheji

Isisombululo esipheleleyo: Iyahambelana nee-incubators kunye nee-colloidal gold analyzers

Isiphelo

I-MilkGuard® 16-in-1 iguqula uhlolo lwee-antibiotics ngokucutha imisebenzi enzima ibe yinkqubo yenyathelo elinye, yemizuzu eli-9 - inika amandla amashishini obisi ukuba anyanzelise ukuthotyelwa kwemithetho ye-zero-residue.
Bukela ividiyo yedemo: