iindaba

I-MilkGuard®16-in-1Rapid Test KitYaziswa: Isikrini se-16Amayeza okubulala iintsholongwaneIiklasi kubisi olukrwada phakathi kweMizuzu eyi-9

Iingenelo ezingundoqo

Uvavanyo oluBanzi oluPhakamileyo

Ngaxeshanye ibhaqa amaqela ama-4 amayeza okubulala iintsholongwane kuzo zonke iintsalela zamachiza ali-16:
• IiSulfonamides (SABT)
• IiQuinolones (TEQL)
• Aminoglycosides (SNK)
• IiMacrolides (SMCF)

Izakhono zokubona eziphambili:

I-Sulfadiazine (8 μg/L), iSulfadimidine (15-20 μg/L)

I-Flofenicol (0.15 μg/L, EU MRL ≤100 μg/L)

I-Monensin (5 μg/L), uColistin (10 μg/L)

Ithobela iChina GB 31650 & EU EC/37/2010 imida yolawulo

Itekhnoloji yokuFunda kabini

Ukutolika okubonakalayo: Thelekisa umgca wovavanyo (umgca we-T) vs. umgca wolawulo (umgca we-C) ukwenzela umnxeba ongalunganga/uPositive ngoko nangoko

Ubungakanani besixhobo: Umhlalutyi wegolide we-Colloidal wenza ingxelo yedatha ngokuzenzekelayo ngokuqinisekiswa kwebhetshi yekhowudi ye-QR

Ukuqukunjelwa kokuSebenza

Inkqubo yemizuzu eyi-9 iyonke: I-3-min i-incubation yesampuli + i-6-min chromatography

Ikhadi elidityanisiweyo elinemicu emi-4: Ukufunyaniswa okufanayo kwinqanaba elinye

Isampulu encinci: 200 μL ubisi olukrwada, akukho nyango lwangaphambili lufunekayo

Umgaqo osemgangathweni

1. ULUNGISELELO LWEMFANELO:

-Linganisa ubisi kwiqondo lobushushu begumbi (homogenized)

- Preheat metal incubator ukuya kwi-45°C (Kuyacetyiswa: Kwinbon Mini-T4)

2. INKQUBO YOVAVANYO:

- Yongeza ubisi lwe-200μL kwi-microwell, xuba nge-pipetting 5x

-Faka ikhadi lovavanyo (iphedi yokufunxa intywiliselwe ngokupheleleyo)

3. UTOLIKO (NGAPHAKATHI KWEMIZUZU emi-2):

-OKUBONWAYO:

Okubi: Ukuqina komgca we-T ≥ Umgca we-C (iintsalela <LOD)

Okuhle: Umgca we-T < Umgca we-C okanye ongabonakaliyo (iintsalela ≥ LOD)

- ISIXHOBO:

Faka ikhadi kwisihlalutyi → Skena ikhowudi yeQR → Ingxelo yobungakanani obuzenzekelayo

Ixabiso loShishino

Ukusebenza kweendleko: Yehlisa iindleko zovavanyo nge-70% vs. single-analyte kits

Ulawulo lomngcipheko: Izilumkiso zakwangoko nge-HPLC-MS/MS yokuqinisekisa inkxaso

Iimeko zesicelo:

Ukuthathwa kobisi olukrwada kwiindawo zokuqokelela

Inkqubo yokujonga umgangatho kwizityalo zobisi

Ukuhlolwa kwendawo yolawulo

Ukuqinisekisa umgangatho

Ubomi beshelufu yeenyanga ezili-12 (2-8°C ugcino olumnyama, UNGAMKHELI)

Ukulandeleka kweBatch: Inani lelothi kunye nomhla wemveliso kwipakethe

Isisombululo esipheleleyo: Iyahambelana ne-incubators kunye nabahlalutyi begolide ye-colloidal

Ukuqukumbela

I-MilkGuard® 16-in-1 iguqula ukuhlolwa kwe-antibiotics ngokujikisa ukuhamba komsebenzi okunzima ukuya kwinqanaba elinye, inkqubo ye-9 yemizuzu - ukuxhobisa amashishini obisi ukuba anyanzelise ukuthotyelwa kwe-zero-residue.
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