Amakholomu e-Immunoaffinity ase-China emboni yokuthola i-Aflatoxin M1 kanye nabakhiqizi

Amakholomu e-Immunoaffinity okuthola i-Aflatoxin M1

Incazelo emfushane:

Amakholomu e-Kwinbon Aflatoxin M1 asetshenziswa ngokuhlanganisa nekhithi yokuhlola ye-HPLC, i-LC-MS, ne-ELISA.

Kungaba ukuhlola i-AFM1 ngobuningi bobisi oluwuketshezi, i-yogurt, ubisi oluyimpuphu, ukudla okukhethekile kokudla, ukhilimu noshizi.

Sithumelele i-imeyili Iziqondiso Zokusebenzisa

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH00902Z
Izakhiwo	Ukuhlolwa kwe-Aflatoxin M1
Indawo Yokuqala	IBeijing, eShayina
I gama le-brand	I-Kwinbon
Usayizi Weyunithi	Ukuhlolwa okungu-25 ngebhokisi ngalinye
Isicelo Sesampula	Lubisi lwe-iquid, i-yogurt, ubisi oluyimpuphu, ukudla okukhethekile kokudla, ukhilimu noshizi
Isitoreji	2-30℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Ukulethwa	Ukushisa kwegumbi

Kudingeka Imishini Nezinto Ezivuselelayo

Imishini

Ama-reagent

Imishini

----Homogenizer -----Umxube weVortex

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni eliphezulu/i-Centrifuge ----Ibhalansi yokuhlaziya (ukungena: 0.01g)

----Ipayipi eliphothuliwe: 10ml ----Injector: 20ml

----Ibhodlela levolumu: 250ml ----Ibhalbhu yepayipi yerabha

----I-Micropipette: 100-1000ul ----I-Glass funnel 50ml

----Izihlungi ze-Microfiber (i-Whatman, 934-AH, Φ11cm, indilinga engu-1.5um)

Ama-reagent

----i-Methanol (AR)

----I-Acetic acid (AR)

----I-Sodium chloride (NACL,AR)

----- Amanzi ahlanjululwe

Izinzuzo zomkhiqizo

Amakholomu e-Kwinbon Inmmunoaffinity asebenzisa i-chromatography ewuketshezi ukuze ahlukanise, ahlanze noma ahlaziye i-Aflatoxin M1. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.

Ukuhlaziywa kwenani le-HPLC kobuthi besikhunta kuyindlela yokuthola evuthiwe. Kokubili i-chromatography yesigaba sangaphambili nesangemuva kuyasebenza. I-HPLC yesigaba sangemuva iyonga imali, kulula ukuyisebenzisa, futhi inobuthi obuphansi be-solvent. Ubuthi obuningi buyancibilika ezigabeni ezihambayo ze-polar bese buhlukaniswa ngamakholomu e-non-polar chromatography, okuhlangabezana nezidingo zokutholwa okusheshayo kobuthi obuningi besikhunta kusampula yobisi. Ama-detector ahlanganisiwe e-UPLC asetshenziswa kancane kancane, ngamamojula okucindezela aphezulu kanye namakholomu amancane e-chromatography yosayizi wezinhlayiya, anganciphisa isikhathi sokusebenza kwesampula, athuthukise ukusebenza kahle kokuhlukaniswa kwe-chromatographic, futhi afinyelele ukuzwela okuphezulu.

I-Beijing Kwinbon inikeza izixazululo eziningi zokuthola ubisi. Ikholomu ye-mycotoxin immunoaffinity ye-Kwinbon inezici eziphezulu, ingakwazi ukubona ngokunembile izinto eziqondiwe, futhi izinzile kakhulu nge-RSD<5%. Umthamo wayo wekholomu kanye nezinga lokutakula nakho kusezingeni eliphezulu embonini.

Ngokucacile okuphezulu, amakholomu e-Kwinbon Aflatoxin M1 angabamba ama-molecule aqondiwe esimweni esimsulwa kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukusebenza. Manje asetshenziswa ngokushesha nangokubanzi ensimini yokudla kanye nokusanhlamvu ukuze kutholakale ama-mycotoxin.