Amakholomu e-Immunoaffinity ase-China ezimboni zokuthola i-Fumonisin kanye nabakhiqizi

Amakholomu e-Immunoaffinity okuthola i-Fumonisin

Incazelo emfushane:

Amakholomu e-Kwinbon Fumonisin asetshenziswa ngokuhlanganisa nekhithi yokuhlola ye-HPLC, i-LC-MS, ne-ELISA. Ifanele okusanhlamvu, ukudla, imithi yaseShayina, njll. futhi ithuthukisa ubumsulwa bamasampula.

Sithumelele i-imeyili Iziqondiso Zokusebenzisa

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH01001Z
Izakhiwo	NgobaI-Fumonisinukuhlolwa
Indawo Yokuqala	IBeijing, eShayina
I gama le-brand	I-Kwinbon
Usayizi Weyunithi	Ukuhlolwa okungu-25 ngebhokisi ngalinye
Isicelo Sesampula	Ukudla, Okusanhlamvu, Okusanhlamvu kanye nezinongo
Isitoreji	2-30℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Ukulethwa	Ukushisa kwegumbi

Kudingeka Imishini Nezinto Ezivuselelayo

Imishini

Ama-reagent

Imishini

----Homogenizer -----Umxube weVortex

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni eliphezulu/i-Centrifuge ----Ibhalansi yokuhlaziya (ukungena: 0.01g)

----Ipayipi eliphothuliwe: 10ml ----Injector: 20ml

----Ibhodlela levolumu: 250ml ----Ibhalbhu yepayipi yerabha

----I-Micropipette: 100-1000ul ----I-Glass funnel 50ml

----Izihlungi ze-Microfiber (i-Whatman, 934-AH, Φ11cm, indilinga engu-1.5um)

Ama-reagent

----i-Methanol (AR)

----I-Acetic acid (AR)

----I-Sodium chloride (NACL,AR)

----- Amanzi ahlanjululwe

Izinzuzo zomkhiqizo

I-Fomonisin (FB) uhlobo lwama-metabolite ancibilika emanzini akhiqizwa yi-fungi ebangela ubuthi. Ingcolisa kakhulu okusanhlamvu nemikhiqizo yayo, futhi inobuthi obukhulu kanye nomthelela womdlavuza emfuyweni ethile, futhi isibe yindawo yocwaningo ngemuva kwe-aflatoxin. Ingabangela umonakalo omkhulu kubantu nasezilwaneni njengoba lesi sidakamizwa sitholakala kabanzi kummbila nakweminye imikhiqizo yezolimo. Muva nje i-fumonisin isibe enye yezinto ezingcolisa kakhulu emhlabeni jikelele. Ucwaningo mayelana nokuvimbela nokulawula i-fumonisin selube yindawo yocwaningo emhlabeni wonke.

I-Wikipedia incoma izindlela ezilandelayo zokuthola;

I-chromatography yezendlalelo ezincane,
Ukuhlolwa kwe-immunosorbent okuxhunywe ku-enzyme
Ukukhanya kwekholomu ye-Immunoaffinity
Ikholomu ye-Immunoaffinity esebenza kahle kakhulu i-chromatography yoketshezi

I-Kwinbon Inmmunoaffinity Columns iyindlela yesithathu, isebenzisa i-liquid chromatography ukuze kuhlukaniswe, kuhlanzwe noma kuhlaziywe i-Fumonisin. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.

Ukuhlaziywa kwenani le-HPLC kobuthi besikhunta kuyindlela yokuthola evuthiwe. Kokubili i-chromatography yesigaba sangaphambili nesangemuva kuyasebenza. I-HPLC yesigaba sangemuva iyonga imali, kulula ukuyisebenzisa, futhi inobuthi obuphansi be-solvent. Ubuthi obuningi buyancibilika ezigabeni ezihambayo ze-polar bese buhlukaniswa ngamakholomu e-non-polar chromatography, okuhlangabezana nezidingo zokutholwa okusheshayo kobuthi obuningi besikhunta kusampula yobisi. Ama-detector ahlanganisiwe e-UPLC asetshenziswa kancane kancane, ngamamojula okucindezela aphezulu kanye namakholomu amancane e-chromatography yosayizi wezinhlayiya, anganciphisa isikhathi sokusebenza kwesampula, athuthukise ukusebenza kahle kokuhlukaniswa kwe-chromatographic, futhi afinyelele ukuzwela okuphezulu.

Ngokucacile okuphezulu, amakholomu e-Kwinbon Fumonisin angabamba ama-molecule aqondiwe esimweni esimsulwa kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukusebenza. Manje asetshenziswa ngokushesha nangokubanzi ensimini yokudla kanye nokusanhlamvu ukuze kutholakale ama-mycotoxins.