Amakholomu e-Immunoaffinity ase-China ezimboni zokuthola i-T-2 mycotoxin kanye nabakhiqizi

Amakholomu e-Immunoaffinity okuthola i-T-2 mycotoxin

Incazelo emfushane:

Amakholomu e-Kwinbon T-2 mycotoxin asetshenziswa ngokuwahlanganisa nekhithi yokuhlola ye-HPLC, i-LC-MS, ne-ELISA.Kungaba ukuhlola inani le-T-2 mycotoxin ukuthola okusanhlamvu nemikhiqizo yokusanhlamvu, isosi yesoya, uviniga, imikhiqizo yesosi, utshwala, ubhontshisi, uwoyela we-rapeseed kanye nemifino, izinto zokusetshenziswa zokudla kanye nemikhiqizo eqediwe, njll.

Sithumelele i-imeyili Iziqondiso Zokusebenzisa

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH01301Z
Izakhiwo	NgobaI-mycotoxin ye-T-2ukuhlolwa
Indawo Yokuqala	IBeijing, eShayina
I gama le-brand	I-Kwinbon
Usayizi Weyunithi	Ukuhlolwa okungu-25 ngebhokisi ngalinye
Isicelo Sesampula	Okusanhlamvu nemikhiqizo yokusanhlamvu, isosi yesoya, uviniga, imikhiqizo yesosi, utshwala, njll.
Isitoreji	2-30℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Ukulethwa	Ukushisa kwegumbi

Kudingeka Imishini Nezinto Ezivuselelayo

Imishini

Ama-reagent

Imishini

----Homogenizer -----Umxube weVortex

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni eliphezulu/i-Centrifuge ----Ibhalansi yokuhlaziya (ukungena: 0.01g)

----Ipayipi eliphothuliwe: 10ml ----Injector: 20ml

----Ibhodlela levolumu: 250ml ----Ibhalbhu yepayipi yerabha

----I-Micropipette: 100-1000ul ----I-Glass funnel 50ml

----Izihlungi ze-Microfiber (i-Whatman, 934-AH, Φ11cm, indilinga engu-1.5um)

Ama-reagent

----i-Methanol (AR)

----I-Acetic acid (AR)

----I-Sodium chloride (NACL,AR)

----- Amanzi ahlanjululwe

Izinzuzo zomkhiqizo

I-T-2 mycotoxin iwumkhiqizo wesikhunta otholakala ngokwemvelo we-Fusarium spp. fungus futhi inobuthi kubantu nakwezinye izilwane. Isimo sezokwelapha esibangelwa yi-alimentary toxic aleukia kanye nezimpawu eziningi ezihlobene nezitho ezahlukahlukene njengesikhumba, umgudu womoya kanye nesisu. Itholakala kuma-T-2 mycotoxins angcolisiwe okusanhlamvu, ukudla kanye nokudla.

I-Kwinbon Inmmunoaffinity Columns iyindlela yesithathu, isebenzisa i-chromatography ewuketshezi ukuze kuhlukaniswe, kuhlanzwe noma kuhlaziywe i-T-2 mycotoxin. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.

Ukuhlaziywa kwenani le-HPLC kobuthi besikhunta kuyindlela yokuthola evuthiwe. Kokubili i-chromatography yesigaba sangaphambili nesangemuva kuyasebenza. I-HPLC yesigaba sangemuva iyonga imali, kulula ukuyisebenzisa, futhi inobuthi obuphansi be-solvent. Ubuthi obuningi buyancibilika ezigabeni ezihambayo ze-polar bese buhlukaniswa ngamakholomu e-non-polar chromatography, okuhlangabezana nezidingo zokutholwa okusheshayo kobuthi obuningi besikhunta kusampula yobisi. Ama-detector ahlanganisiwe e-UPLC asetshenziswa kancane kancane, ngamamojula okucindezela aphezulu kanye namakholomu amancane e-chromatography yosayizi wezinhlayiya, anganciphisa isikhathi sokusebenza kwesampula, athuthukise ukusebenza kahle kokuhlukaniswa kwe-chromatographic, futhi afinyelele ukuzwela okuphezulu.

Ngokucacile okuphezulu, amakholomu e-Kwinbon T-2 mycotoxin angabamba ama-molecule aqondiwe esimweni esimsulwa kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukusebenza. Manje asetshenziswa ngokushesha nangokubanzi ensimini yokudla kanye nokusanhlamvu ukuze kutholakale i-mycotoxins.