Amakholomu e-Immunoaffinity ase-China ezimboni zokuthola i-Zearalenone kanye nabakhiqizi

Amakholomu e-Immunoaffinity okuthola i-Zearalenone

Incazelo emfushane:

Amakholomu e-Kwinbon Zearalenone asetshenziswa ngokuhlanganisa nekhithi yokuhlola ye-HPLC, LC-MS, ELISA. Kungaba ukuhlolwa kwenani kwe-AFB1 kokusanhlamvu nemikhiqizo yokusanhlamvu, isosi yesoya, uviniga, imikhiqizo yesosi, utshwala, ubhontshisi, uwoyela we-rapeseed kanye nemifino, izinto zokusetshenziswa zokudla kanye nemikhiqizo eqediwe, njll.

Sithumelele i-imeyili Iziqondiso Zokusebenzisa

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH00304Z
Izakhiwo	NgobaI-Zearalenoneukuhlolwa
Indawo Yokuqala	IBeijing, eShayina
I gama le-brand	I-Kwinbon
Usayizi Weyunithi	Ukuhlolwa okungu-25 ngebhokisi ngalinye
Isicelo Sesampula	Okusanhlamvu nemikhiqizo yokusanhlamvu, isosi yesoya, uviniga, imikhiqizo yesosi, utshwala, isoya, uwoyela we-rapeseed kanye nemifino, izinto zokusetshenziswa zokudla kanye nemikhiqizo eqediwe, njll.
Isitoreji	2-30℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Ukulethwa	Ukushisa kwegumbi

Kudingeka Imishini Nezinto Ezivuselelayo

Imishini

Ama-reagent

Imishini

----Homogenizer -----Umxube weVortex

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni eliphezulu/i-Centrifuge ----Ibhalansi yokuhlaziya (ukungena: 0.01g)

----Ipayipi eliphothuliwe: 10ml ----Injector: 20ml

----Ibhodlela levolumu: 250ml ----Ibhalbhu yepayipi yerabha

----I-Micropipette: 100-1000ul ----I-Glass funnel 50ml

----Izihlungi ze-Microfiber (i-Whatman, 934-AH, Φ11cm, indilinga engu-1.5um)

Ama-reagent

----i-Methanol (AR)

----I-Acetic acid (AR)

----I-Sodium chloride (NACL,AR)

----- Amanzi ahlanjululwe

Izinzuzo zomkhiqizo

I-Zearalenone (ZEN), eyaziwa nangokuthi i-RAL kanye ne-F-2 mycotoxin, iyi-metabolite enamandla ye-estrogenic ekhiqizwayo. Ihlala isikhathi eside ekushiseni futhi itholakala emhlabeni wonke ezitshalweni eziningana zokusanhlamvu, njengommbila, ibhali, i-oats, ukolweni, ilayisi kanye namabele.

I-Zearalenone iyi-toxin eyinhloko ebopha kuma-receptor e-estrogen, okubangela ukungazali, ukukhipha isisu noma ezinye izinkinga zokuzala, ikakhulukazi ezingulubeni.

I-Wikipedia incoma izindlela ezilandelayo zokuthola;

I-chromatography yezendlalelo ezincane,
Ukuhlolwa kwe-immunosorbent okuxhunywe ku-enzyme
Ukukhanya kwekholomu ye-Immunoaffinity
Ikholomu ye-Immunoaffinity esebenza kahle kakhulu i-chromatography yoketshezi

I-Kwinbon Inmmunoaffinity Columns iyindlela yesithathu, isebenzisa i-chromatography ewuketshezi ukuze kuhlukaniswe, kuhlanzwe noma kuhlaziywe i-Zearalenone. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.

Ukuhlaziywa kwenani le-HPLC kobuthi besikhunta kuyindlela yokuthola evuthiwe. Kokubili i-chromatography yesigaba sangaphambili nesangemuva kuyasebenza. I-HPLC yesigaba sangemuva iyonga imali, kulula ukuyisebenzisa, futhi inobuthi obuphansi be-solvent. Ubuthi obuningi buyancibilika ezigabeni ezihambayo ze-polar bese buhlukaniswa ngamakholomu e-non-polar chromatography, okuhlangabezana nezidingo zokutholwa okusheshayo kobuthi obuningi besikhunta kusampula yobisi. Ama-detector ahlanganisiwe e-UPLC asetshenziswa kancane kancane, ngamamojula okucindezela aphezulu kanye namakholomu amancane e-chromatography yosayizi wezinhlayiya, anganciphisa isikhathi sokusebenza kwesampula, athuthukise ukusebenza kahle kokuhlukaniswa kwe-chromatographic, futhi afinyelele ukuzwela okuphezulu.

Ngokucacile okuphezulu, amakholomu e-Kwinbon Zearalenone angabamba ama-molecule aqondiwe esimweni esimsulwa kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukusebenza. Manje asetshenziswa ngokushesha nangokubanzi ensimini yokudla kanye nokusanhlamvu ukuze kutholakale ama-mycotoxins.