Umugqa wokuhlola izinsalela ze-Aflatoxin M1isekelwe esimisweni sokuvimbela ukuncintisana kwe-immunochromatography, i-aflatoxin M1 esesilinganisweni ibopha ku-antibody ethile ye-monoclonal ebhalwe ngegolide enqubweni yokugeleza, okuvimbela ukubopha kwe-antibody kanye nokuhlanganiswa kwe-antigen-BSA emkhawulweni wokutholwa kwe-membrane ye-NC, ngaleyo ndlela kuholele ekushintsheni kokujula kombala womugqa we-T; futhi kungakhathaliseki ukuthi isampula iqukethe yini into ezotholakala, umugqa we-C uzofakwa umbala, ukuze ubonise ukuthi ukuhlolwa kuyasebenza. Imichilo yokuhlola ye-Aflatoxin M1 ingafaniswa ne-umfundiukukhipha idatha yokuhlolwa nokuhlaziya idatha ukuze kutholakale umphumela wokugcina wokuhlolwa.
Imichilo yokuhlola i-Aflatoxin M1 residue ifanelekela ukunqunywa kwekhwalithi ye-aflatoxin M1 kumasampula obisi aluhlaza nalawa aphekiwe. Umkhawulo wokuthola u-0.5 ppb, ukuhlolwa kukhombisa ukuthi akunawo umphumela omuhle nge-500 μg/L ye-sulfamethazine, i-norfloxacin, i-lincomycin, i-spectinomycin, i-gentamicin, i-streptomycin kanye neminye imithi, ukuhlolwa kukhombisa ukuthi kunemiphumela emihle nge-5 μg/L ye-Aflatoxin B1.
Isikhathi sokuthunyelwe: Ephreli-01-2024