izindaba

I-MilkGuard®16-ku-1Ikhithi Yokuhlola EsheshayoKwethulwe: Isikrini 16AmagciwaneAmakilasi Obisi Oluluhlaza Ngaphakathi Kwamaminithi angu-9

Izinzuzo Eziyinhloko

Ukuhlolwa Okuphelele Kokudlula Okuphezulu

Ngesikhathi esifanayo ithola amaqembu ama-antibiotic angu-4 kuzo zonke izinsalela zemithi engu-16:
• I-Sulfonamides (SABT)
• Ama-Quinolones (TEQL)
• Ama-Aminoglycosides (SNK)
• Ama-Macrolides (SMCF)

Amandla okuthola ukhiye:

I-Sulfadiazine (8 μg/L), i-Sulfadimidine (15-20 μg/L)

I-Flofenicol (0.15 μg/L, EU MRL ≤100 μg/L)

I-Monensin (5 μg/L), i-Colistin (10 μg/L)

Ithobelana nemikhawulo yokulawula yase-China GB 31650 kanye ne-EU EC/37/2010

I-Dual-Readout Technology

Ukuhumusha okubonakalayo: Qhathanisa umugqa wokuhlola (umugqa we-T) vs. ulayini wokulawula (umugqa we-C) ukuze uthole ikholi esheshayo ethi Negative/Positive

Ubuningi bensimbi: Umhlaziyi wegolide we-Colloidal wenza ngokuzenzakalelayo ukubika idatha ngokuqinisekiswa kwenqwaba yekhodi ye-QR

Ukugeleza Kokusebenza Okulula

Inqubo yemizuzu engu-9 iyonke: 3-minthi incubation isampula + 6-min chromatography

Ikhadi lemicu emi-4 elihlanganisiwe: Ukutholwa okufanayo esinyathelweni esisodwa

Isampula elincane: 200 μL ubisi olungaphekiwe, akukho ukwelashwa kwangaphambili okudingekayo

Iphrothokholi Ejwayelekile

1. UKULUNGISELELA ESAYIMPELA:

- Linganisa ubisi ekamelweni lokushisa (homogenized)

- Preheat metal incubator ukuya ku-45°C (Kunconyiwe: Kwinbon Mini-T4)

2. INKQUBO YOKUHLOLA:

- Faka ubisi lwe-200μL ku-microwell, hlanganisa ngokufaka ipayipi 5x

- Faka ikhadi lokuhlola (iphedi elimunca licwiliswe ngokuphelele)

3. UKUTOLIZEKA (NGAKA-2 IMIZUZU):

- OKUBONWAYO:

Okubi: Ukuqina komugqa we-T ≥ umugqa we-C (izinsalela <LOD)

Okuhle: Umugqa we-T < C-line noma ongabonakali (izinsalela ≥ LOD)

- Instrument:

Faka ikhadi kusihlaziyi → Skena ikhodi ye-QR → Umbiko womthamo ozenzakalelayo

Inani Lomkhakha

Ukusebenza kahle kwezindleko: Yehlisa izindleko zokuhlola ngo-70% uma kuqhathaniswa namakhithi e-single-analyte

Ukulawula ubungozi: Izaziso zangaphambi kwesikhathi ezinokwesekwa kokuqinisekisa kwe-HPLC-MS/MS

Izimo zohlelo lokusebenza:

Ukudla ubisi olungaphekiwe ezindaweni zokuqoqa

Ukuhlolwa kwekhwalithi okungaphakathi kwenqubo ezitshalweni zobisi

Ukuhlolwa kwenkundla yokulawula

Ukuqinisekisa ikhwalithi

Impilo yeshelufu yezinyanga eziyi-12 (isitoreji esimnyama esingu-2-8°C, UNGAMIMI)

Ukulandeleka kweqoqo: Inombolo yelothi nosuku lokukhiqiza emaphaketheni

Isixazululo esigcwele: Ihambisana nama-incubator kanye nabahlaziyi begolide be-colloidal

Isiphetho

I-MilkGuard® 16-in-1 iguqula ukuhlolwa kwemithi elwa namagciwane ngokufingqa ukuhamba komsebenzi okuyinkimbinkimbi kube isinyathelo esisodwa, inqubo yemizuzu engu-9 - inika amandla izimboni zobisi ukuthi ziphoqeleke ukuthobela imithetho eyinsalela.
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