Litšiea tsa China tsa Immunoaffinity bakeng sa fektheri ea ho lemoha ea Column Vomitoxin le Zearlenone 2 ho 1 le bahlahisi

Likholomo tsa Immunoaffinity bakeng sa ho lemoha Column Vomitoxin le Zearlenone 2 ho 1

Tlhaloso e Khutšoanyane:

Likholomo tsa Kwinbon Vomitoxin le Zearlenone li sebelisoa ka ho kopanya le kit ea teko ea HPLC, LC-MS, le ELISA.E ka ba teko ea bongata ea Vomitoxin le Zearlenone bakeng sa lijo-thollo, lijo, meriana ea Chaena, jj. le ho ntlafatsa bohloeki ba lisampole.

Romella imeile ho rona Litaelo tsa Tšebeliso

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Litlhaloso tsa sehlahisoa

Nomoro ea katse.	KH02102Z
Matlo	Bakeng sa Vomitoxin le Zearlenonetlhahlobo
Sebaka sa Tšimoloho	Beijing, Chaena
Lebitso la Letšoao	Kwinbon
Boholo ba Yuniti	Liteko tse 25 ka lebokose
Sesebelisoa sa Mohlala	Fepa, Lijo-thollo, Lijo-thollo le Linoko
Polokelo	2-30℃
Nako ea ho phela	Likhoeli tse 12
Thomello	Mocheso oa kamore

Lisebelisoa le Li-reagent tse Hlokahalang

Lisebelisoa

Li-reagent

Lisebelisoa

----Homogenizer ----Motsoako oa Vortex

----Botlolo ea mohlala ----Silindara ea ho lekanya: 10ml, 100ml

----Pampiri ea sefe/Centrifuge ea boleng bo holimo ----Tekanyo ea tlhahlobo (inductance: 0.01g)

----Pipette e koetlisitsoeng: 10ml -----Injector: 20ml

----Folaskete ea volumetric: 250ml ----Lebone la rabara la pipette

----Micropipette: 100-1000ul ----Funnel ea khalase 50ml

----Li-Filter tsa Microfiber (Whatman, 934-AH, Φ11cm, selikalikoe sa 1.5um)

Li-reagent

----Methanol (AR)

----Acetic acid (AR)

----Sodium chloride (NACL,AR)

-----Metsi a hloekisitsoeng

Melemo ea sehlahisoa

Likholomo tsa Kwinbon Inmmunoaffinity li sebelisa chromatography ea metsi bakeng sa karohano, tlhoekiso kapa tlhahlobo e khethehileng ea Aflatoxin Total. Hangata likholomo tsa Kwinbon li kopanngoa le HPLC.
Antibody ea monoclonal khahlanong le Aflatoxin Total e hokahane le mecha ea ho kopanya e kholomong. Li-mycotoxin tse sampoleng lia ntšoa, lia sefshoa ebe lia hlapolloa. Etsa hore tharollo ea ho ntša sampole e fete kholomong ea Aflatoxin Total. Masalla a Aflatoxin (B1, B2, G1, G2) a kopanngoa le antibody ka thoko kholomong, tharollo ea ho hlatsoa e tlosa litšila tse sa kopanngoang. Qetellong, ho sebelisoa joala ba methyl ho tlosa Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2.
Ka ho hlaka ho hoholo, likholomo tsa Kwinbon AFT li ka tšoasa limolek'hule tse reretsoeng boemong bo hloekileng haholo. Hape likholomo tsa Kwinbon li phalla ka potlako, ho bonolo ho li sebetsa. Hona joale e sebelisoa ka potlako le ka bongata tšimong ea lijo le lijo-thollo bakeng sa ho thetsa chefo ea mycotoxins.