Litšiea tsa China tsa Immunoaffinity bakeng sa fektheri ea ho lemoha mycotoxin ea T-2 le bahlahisi

Likholomo tsa immunoaffinity bakeng sa ho lemoha chefo ea mycotoxin ea T-2 Setšoantšo se Hlahisitsoeng

Likholomo tsa immunoaffinity bakeng sa ho lemoha chefo ea T-2 mycotoxin

Tlhaloso e Khutšoanyane:

Likholomo tsa mycotoxin tsa Kwinbon T-2 li sebelisoa ka ho kopanya le kit ea teko ea HPLC, LC-MS, le ELISA.E ka ba teko ea bongata ea mycotoxin ea T-2 bakeng sa lijo-thollo le lihlahisoa tsa lijo-thollo, soy sauce, asene, lihlahisoa tsa soso, joala, soya, oli ea peo ea rapeseed le ea meroho, fepa thepa e tala le lihlahisoa tse felileng, jj.

Romella imeile ho rona Litaelo tsa Tšebeliso

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Litlhaloso tsa sehlahisoa

Nomoro ea katse.	KH01301Z
Matlo	Bakeng saT-2 mycotoxintlhahlobo
Sebaka sa Tšimoloho	Beijing, Chaena
Lebitso la Letšoao	Kwinbon
Boholo ba Yuniti	Liteko tse 25 ka lebokose
Sesebelisoa sa Mohlala	Lihlahisoa tsa lijo-thollo le tsa lijo-thollo, soso ea soya, asene, lihlahisoa tsa soso, joala, jj.
Polokelo	2-30℃
Nako ea ho phela	Likhoeli tse 12
Thomello	Mocheso oa kamore

Lisebelisoa le Li-reagent tse Hlokahalang

Lisebelisoa

Li-reagent

Lisebelisoa

----Homogenizer ----Motsoako oa Vortex

----Botlolo ea mohlala ----Silindara ea ho lekanya: 10ml, 100ml

----Pampiri ea sefe/Centrifuge ea boleng bo holimo ----Tekanyo ea tlhahlobo (inductance: 0.01g)

----Pipette e koetlisitsoeng: 10ml -----Injector: 20ml

----Folaskete ea volumetric: 250ml ----Lebone la rabara la pipette

----Micropipette: 100-1000ul ----Funnel ea khalase 50ml

----Li-Filter tsa Microfiber (Whatman, 934-AH, Φ11cm, selikalikoe sa 1.5um)

Li-reagent

----Methanol (AR)

----Acetic acid (AR)

----Sodium chloride (NACL,AR)

-----Metsi a hloekisitsoeng

Melemo ea sehlahisoa

T-2 mycotoxin ke sehlahisoa sa tlhaho sa hlobo sa Fusarium spp. fungus 'me e chefo ho batho le liphoofolo tse ling. Boemo ba kliniki boo e bo bakang ke aleukia e chefo ea lijo le matšoao a mangata a amanang le litho tse fapaneng joalo ka letlalo, moea le mpa. E fumaneha ka har'a li-mycotoxin tsa T-2 tse silafetseng, lijo le lijo.

Likholomo tsa Kwinbon Inmmunoaffinity ke mokhoa oa boraro, o sebelisa chromatography ea metsi bakeng sa karohano, tlhoekiso kapa tlhahlobo e khethehileng ea mycotoxin ea T-2. Hangata likholomo tsa Kwinbon li kopantsoe le HPLC.

Tlhahlobo ea HPLC ea chefo ea fungus ke mokhoa oa ho lemoha o hōlileng tsebong. Ka bobeli chromatography ea mohato o ka pele le o ka morao lia sebetsa. HPLC ea mohato o ka morao e theko e tlaase, e bonolo ho e sebelisa, 'me e na le chefo e fokolang ea solvent. Bongata ba chefo boa qhibiliha mekhahlelong e tsamaeang ea polar ebe bo aroloa ke likholomo tsa chromatography tse seng tsa polar, e leng se fihlelang litlhoko tsa ho lemoha ka potlako chefo e mengata ea fungus sampole ea lebese. Lisebelisoa tse kopaneng tsa UPLC li ntse li sebelisoa butle-butle, ka li-module tse nang le khatello e phahameng le likholomo tse nyane tsa chromatography tsa boholo ba likaroloana, tse ka khutsufatsang nako ea ho sebetsa ha sampole, tsa ntlafatsa katleho ea karohano ea chromatographic, le ho fihlela kutloisiso e phahameng.

Ka ho hlaka ho hoholo, likholomo tsa mycotoxin tsa Kwinbon T-2 li ka tšoasa limolek'hule tse reretsoeng boemong bo hloekileng haholo. Hape likholomo tsa Kwinbon li phalla ka potlako, ho bonolo ho li sebetsa. Hona joale e sebelisoa ka potlako le ka bongata tšimong ea lijo le lijo-thollo bakeng sa ho qhekella li-mycotoxin.