Litšiea tsa China tsa Immunoaffinity bakeng sa fektheri le bahlahisi ba ho lemoha Vomitoxin

Likholomo tsa immunoaffinity bakeng sa ho lemoha Vomitoxin

Tlhaloso e Khutšoanyane:

Likholomo tsa Kwinbon Vomitoxin li sebelisoa ka ho kopanya le kit ea teko ea HPLC, LC-MS, le ELISA.E ka ba teko ea bongata ea AFB1 bakeng sa lijo-thollo, linate tsa peanut le lihlahisoa tsa tsona, oli ea meroho le mafura, lihlahisoa tsa linate, soy sauce, asene, meriana ea Chaena, linoko le tee.

Romella imeile ho rona Litaelo tsa Tšebeliso

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Litlhaloso tsa sehlahisoa

Nomoro ea katse.	KH00203Z
Matlo	Bakeng sa liteko tsa Vomitoxin
Sebaka sa Tšimoloho	Beijing, Chaena
Lebitso la Letšoao	Kwinbon
Boholo ba Yuniti	Liteko tse 25 ka lebokose
Sesebelisoa sa Mohlala	Lijo-thollo, linate le lihlahisoa tsa tsona, oli ea meroho le mafura, lihlahisoa tsa linate, soya sauce, asene, meriana ea Chaena, linoko le tee
Polokelo	2-30℃
Nako ea ho phela	Likhoeli tse 12
Thomello	Mocheso oa kamore

Lisebelisoa le Li-reagent tse Hlokahalang

Lisebelisoa

Li-reagent

Lisebelisoa

----Homogenizer ----Motsoako oa Vortex

----Botlolo ea mohlala ----Silindara ea ho lekanya: 10ml, 100ml

----Pampiri ea sefe/Centrifuge ea boleng bo holimo ----Tekanyo ea tlhahlobo (inductance: 0.01g)

----Pipette e koetlisitsoeng: 10ml -----Injector: 20ml

----Folaskete ea volumetric: 250ml ----Lebone la rabara la pipette

----Micropipette: 100-1000ul ----Funnel ea khalase 50ml

----Li-Filter tsa Microfiber (Whatman, 934-AH, Φ11cm, selikalikoe sa 1.5um)

Li-reagent

----Methanol (AR)

----Acetic acid (AR)

----Sodium chloride (NACL,AR)

-----Metsi a hloekisitsoeng

Melemo ea sehlahisoa

Vomitoxin, e tsejoang hape e le deoxynivalenol (DON), ke epoxy-sesquiterpenoid. E hlaha haholo-holo lijo-thollong tse kang koro, harese, oats, rye le poone, 'me hangata ha e hlahe raeseng, mabele le triticale, e leng se bakang ho hlatsa le ho hloka takatso ea lijo liphoofolong 'me ho ka 'na ha lebisa ho nyekeloa ke pelo le ho hlatsa bathong.

Wikipedia e khothaletsa mekhoa e latelang ea ho lemoha;

Kromatography ea lera le lesesaane,
Teko ea immunosorbent e hokahaneng le enzyme
Phatsimo ea kholomo ea immunoaffinity
Chromatography ea metsi e sebetsang hantle ea Immunoaffinity

Likholomo tsa Kwinbon Inmmunoaffinity ke mokhoa oa boraro, o sebelisa chromatography ea metsi bakeng sa karohano, tlhoekiso kapa tlhahlobo e khethehileng ea Vomitoxin. Hangata likholomo tsa Kwinbon li kopantsoe le HPLC.

Tlhahlobo ea HPLC ea chefo ea fungus ke mokhoa oa ho lemoha o hōlileng tsebong. Ka bobeli chromatography ea mohato o ka pele le o ka morao lia sebetsa. HPLC ea mohato o ka morao e theko e tlaase, e bonolo ho e sebelisa, 'me e na le chefo e fokolang ea solvent. Bongata ba chefo boa qhibiliha mekhahlelong e tsamaeang ea polar ebe bo aroloa ke likholomo tsa chromatography tse seng tsa polar, e leng se fihlelang litlhoko tsa ho lemoha ka potlako chefo e mengata ea fungus sampole ea lebese. Lisebelisoa tse kopaneng tsa UPLC li ntse li sebelisoa butle-butle, ka li-module tse nang le khatello e phahameng le likholomo tse nyane tsa chromatography tsa boholo ba likaroloana, tse ka khutsufatsang nako ea ho sebetsa ha sampole, tsa ntlafatsa katleho ea karohano ea chromatographic, le ho fihlela kutloisiso e phahameng.

Ka ho hlaka ho hoholo, likholomo tsa Kwinbon Vomitoxin li ka tšoasa limolek'hule tse reretsoeng boemong bo hloekileng haholo. Hape likholomo tsa Kwinbon li phalla ka potlako, ho bonolo ho li sebetsa. Hona joale li sebelisoa ka potlako le ka bongata tšimong ea lijo le lijo-thollo bakeng sa ho qhekella li-mycotoxin.