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Elisa Test Kit of AOZ Featured Image
  • Elisa Test Kit of AOZ
  • Elisa Test Kit of AOZ

Elisa Test Kit of AOZ

Inkcazelo emfutshane:

Le kit ingasetyenziselwa uhlalutyo lobungakanani kunye nomgangatho we-AOZ intsalela kwizicubu zezilwanyana (inkukhu, inkomo, ihagu, njl njl), ubisi , ubusi kunye namaqanda.
Uhlalutyo lwentsalela yamachiza e-nitrofuran kufuneka lusekelwe ekufumaneni i-metabolites ebophelelwe kwizicubu ze-nitrofuran iziyobisi zabazali, ezibandakanya i-Furazolidone metabolite (AOZ), i-Furaltadone metabolite (AMOZ), i-Nitrofurantoin metabolite (AHD) kunye ne-Nitrofurazone metabolite (SEM).
Xa kuthelekiswa neendlela zechromatographic, ikhithi yethu ibonisa inzuzo enkulu malunga nobuntununtunu, umda wokubona, izixhobo zobugcisa kunye nexesha elifunekayo.


Imikhomba-ndlela yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

I-Nitrofurans zi-antibiotics zokwenziwa ezibanzi, ezihlala zisetyenziswa kwimveliso yezilwanyana ngenxa yeempawu zayo ezibalaseleyo zokulwa ne-antibacterial kunye ne-pharmacokinetic.
Zaziphinde zasetyenziswa njengabakhuthazi bokukhula kwihagu, iinkukhu kunye nemveliso yasemanzini.Kwizifundo zexesha elide kunye nezilwanyana zaselebhu zibonise ukuba amachiza omzali kunye ne-metabolites yawo abonise iimpawu ze-carcinogenic kunye ne-mutagenic.Izidakamizwa ze-nitrofuran i-furaltadone, i-nitrofurantoin kunye ne-nitrofurazone zavalwa ukusetyenziswa kwimveliso yezilwanyana zokutya kwi-EU kwi-1993, kwaye ukusetyenziswa kwe-furazolidone kwakungavunyelwe kwi-1995.

Iinkcukacha

1.Elisa Test Kit of AOZ

2.Ikati.A008-96 Wells

3.Izixhobo zeKit
● I-Microtiter plate eqatywe nge-antigen, amaqula angama-96
● Izisombululo eziqhelekileyo (iibhotile ezi-6, 1ml/ibhotile)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
● Ulawulo lomgangatho wokuspiking : (1ml/ibhotile)............................................. .............100ppb
● Enzyme conjugate concentrate 1.5ml............................................ ..….….ikepusi ebomvu
● Isisombululo se-antibody sigxininiswe 0.8ml……………………………………………………
● I-Substrate A 7ml ………………………………………………… .....................................…..ikepusi emhlophe
● I-Substrate B7ml……………………………………………… ....................…..…..ikepusi ebomvu
● Misa isisombululo 7ml ……………………………………………………………
● 20×isisombululo esigxininisiweyo sokuhlamba 40ml ………………………….……
● 2×isisombululo esigxininisiweyo sotsalo 60ml………………………..…………….blue cap
● 2-Nitrobenzaldehyde 15.1mg……………………………………………………ikepusi emnyama

4.Uvakalelo, ukuchaneka nokuchaneka
Uvakalelo: 0.025ppb
Umda wokuchongwa……………..……………………………………
Ukuchaneka:
Izicubu zezilwanyana (izihlunu nesibindi)……….……………75±15%
Ubusi……………………………………………………..90±20%
Iqanda……………………………………………………..…90±20%
Ubisi……………………………………………………..…90±10%
Ukuchaneka: I-CV yekhithi ye-ELISA ingaphantsi kwe-10%.

5.Ireyithi enqamlezileyo
I-Furazolidone metabolite (AOZ)……………………………………………..100%
I-Furaltadone metabolite (AMOZ)…………………………………………<0.1%
I-Nitrofurantoin metabolite (AHD)………………………………………<0.1%
I-Nitrofurazone metabolite (SEM)…………………………………………<0.1%
IFurazolidone……………………………………………………….…..…16.3%
Furaltadone………………………………………………………….…<1%
INitrofurantoin…………………………………………………….….…<1%
INitrofurazone…………………………………………………………..…<1%

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