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Elisa Test Kit ya Aflatoxin B1 Chithunzi Chowonetsedwa
  • Elisa Test Kit ya Aflatoxin B1
  • Elisa Test Kit ya Aflatoxin B1
  • Elisa Test Kit ya Aflatoxin B1

Elisa Test Kit ya Aflatoxin B1

Kufotokozera Kwachidule:

Kuchuluka kwa ma aflatoxins kumabweretsa chiphe (aflatoxicosis) chomwe chingakhale chowopseza moyo, nthawi zambiri chifukwa cha kuwonongeka kwa chiwindi.

Aflatoxin B1 ndi aflatoxin yopangidwa ndi Aspergillus flavus ndi A. parasiticus.Ndi carcinogen yamphamvu kwambiri.Mphamvu ya carcinogenic iyi imasiyanasiyana kutengera mitundu ndi ena, monga makoswe ndi anyani, omwe amawoneka kuti ndi owopsa kwambiri kuposa ena.Aflatoxin B1 ndizovuta kwambiri muzakudya zosiyanasiyana kuphatikiza mtedza, ufa wa thonje, chimanga, ndi mbewu zina;komanso zakudya za ziweto.Aflatoxin B1 imatengedwa kuti ndi aflatoxin wapoizoni kwambiri ndipo imakhudzidwa kwambiri ndi kansa ya hepatocellular carcinoma (HCC) mwa anthu.Zitsanzo zingapo ndi njira zowunikira kuphatikiza thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), mass spectrometry, ndi enzyme-linked immunosorbent assay (ELISA), mwa zina, zagwiritsidwa ntchito kuyesa kuipitsidwa kwa aflatoxin B1 muzakudya. .Malinga ndi bungwe la Food and Agriculture Organisation (FAO), kuchuluka kwa aflatoxin B1 padziko lonse lapansi komwe kumaloledwa ndi 1-20 μg/kg m'zakudya, ndi 5-50 μg/kg pazakudya za ng'ombe mu 2003.


Malangizo Ogwiritsa Ntchito

Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Za

Chidachi chitha kugwiritsidwa ntchito poyesa kuchuluka komanso kuchuluka kwa aflatoxin B1 mumafuta odyedwa, chiponde, tirigu, msuzi wa soya, viniga ndi chakudya (zakudya zosaphika, zosakaniza za batch ndi zinthu zokhazikika). kusanthula zida.

Izi zimatengera mpikisano wosalunjika wa ELISA, womwe umakhala wofulumira, wolondola komanso womvera poyerekeza ndi kusanthula kwa zida wamba.Zimangofunika mphindi 45 zokha pakugwira ntchito kumodzi, zomwe zingachepetse kwambiri cholakwika cha ntchito komanso kulimba kwa ntchito.

Zida zamagulu

• Microtiter plateprecoated ndi antigen, 96 zitsime

• Standard Solution × 6botolo(1ml/botolo)

0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb

• Enzyme conjugate 7ml………………………………………………………………..…………kapu yofiira

• Antibody solution7ml................................................................. .................………kapu yobiriwira

• Substrate A 7ml………………………………………………………………….………………kapu yoyera

• Gawo B 7ml………………………………………………………………………….…………kapu yofiyira

• Stop solution 7ml……….……………………………………………………………..………kapu yachikasu

• 20×20xconcentrated wash solution 40ml ………………………………………………kapu yowonekera

• 2× 50ml yankho lokhazikika la m'zigawo …………………………………………………kapu yabuluu

Sensitivity, kulondola ndi kulondola

Kukhudzika:0.05ppb

Malire ozindikira

Mafuta ophikira amafuta .......................................... .......................................................................0.1ppb

Mtedza.................................................................. .................................................. .......................0.2ppb

Cereal................................................. .................................................. .....................0.05ppb

Kulondola

Mafuta ophikira amafuta .......................................... .................................................................80±15%

Mtedza.................................................................. .................................................. ....................80±15%

Cereal................................................. .................................................. ....................80±15%

Kulondola

Kusiyanasiyana kwa zida za ELISA ndizochepera 10%.

Cross Rate

Aflatoxin B1················100%

Aflatoxin B2 ·····················81 .3%

Aflatoxin G1 · ····················62%

Aflatoxin G2 · ·····················22.3%

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