umkhiqizo

Mayelana

Le khithi ingasetshenziselwa ukuhlaziywa kwekhwalithi nenani le-aflatoxin B1 emafutheni adliwayo, amakinati, okusanhlamvu okusanhlamvu, isoso yesoya, uviniga kanye nokudla (okuphakelayo okuluhlaza , inqwaba yezinto ezixutshwe nezinto ezigxilile). ukuhlaziywa kwezinsimbi.

Lo mkhiqizo usekelwe ku-ELISA yokuncintisana engaqondile, esheshayo, enembayo futhi ezwelayo uma iqhathaniswa nokuhlaziywa kwezinsimbi okuvamile.Idinga kuphela imizuzu engama-45 ekusebenzeni okukodwa, okunganciphisa kakhulu iphutha lokusebenza kanye nokuqina komsebenzi.

Izingxenye zekhithi

• I-Microtiter plateprecoated ne-antigen, imithombo engama-96

• Isixazululo Esijwayelekile ×6ibhodlela(1ml/ibhodlela)

0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb

• I-Enzyme conjugate 7ml……………………………………………………………..……………ikepisi elibomvu

• Isixazululo se-antibody7ml................................................................. .......................………ikepisi eliluhlaza

• I-Substrate A 7ml……………………………………………………………….…………………ikepisi elimhlophe

• I-Substrate B 7ml……………….…………………………………………………….…………ikepisi elibomvu

• Isixazululo sokumisa 7ml……….…………………………………………………………..………ikepisi eliphuzi

• 20×isixazululo sokugeza esigxilile 40ml ………………………………………………ikepisi esobala

• 2×isixazululo sokukhipha okugxilisiwe 50ml……………………………………………………ikepisi eliluhlaza okwesibhakabhaka

Ukuzwela, ukunemba nokunemba

Ukuzwela:0.05ppb

Umkhawulo wokutholwa

Isampula yamafutha adliwayo .......................................... .........................................................................0.1ppb

I-Peanut................................................. .................................................. .......................0.2ppb

Okusanhlamvu................................................. .................................................. .....................0.05ppb

Ukunemba

Isampula yamafutha adliwayo .......................................... .................................................................80±15%

I-Peanut................................................. .................................................. ....................80±15%

Okusanhlamvu................................................. .................................................. ....................80±15%

Ukunemba

I-Coefficient ehlukile yekhithi ye-ELISA ingaphansi kuka-10%.

I-Cross Rate

I-Aflatoxin B1·················100%

I-Aflatoxin B2 ······················81 .3%

I-Aflatoxin G1 ······················62%

I-Aflatoxin G2 ······················22.3%