umkhiqizo

Mayelana

Le khithi ingasetshenziswa ekuhlaziyeni inani nekhwalithi ye-ochratoxin A ekuphakeleni.Ungumkhiqizo omusha wokutholwa kwezinsalela zomuthi ngokusekelwe kubuchwepheshe be-ELISA, obiza kuphela amaminithi angu-30 ekusebenzeni ngakunye futhi onganciphisa kakhulu amaphutha okusebenza kanye nokuqina komsebenzi.Le khithi isuselwe kubuchwepheshe bokuncintisana obungaqondile be-ELISA.Imithombo ye-microtiter ihlanganiswe ne-antigen ehlangene.I-Ochratoxin A kusampula iqhudelana ne-antigen ehlanganiswe epuleti le-microtiter ye-antibody eyengezwe .Ngemuva kokwengezwa kwe-enzyme conjugate, i-substrate ye-TMB isetshenziselwa ukukhombisa umbala.Ukungabi bikho kwesampula kuhlobene kabi nensalela ye-o chratoxin A ekuyo, ngemva kokuqhathanisa ne-Standard Curve, ephindaphindwa izici zokuhlanjululwa,OI-chratoxin Inani kusampula lingabalwa.

Izingxenye Zekhithi

• Ipuleti leMicrotiter elinemithombo engama-96 embozwe nge-antigen

•Sizixazululo ezijwayelekile (amabhodlela ayi-6: 1ml/ibhodlela)

0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb

• I-enzymehlanganisa7ml……………………………………………………………………..…………..…..ikepisi elibomvu

• Isixazululo se-antibody10ml…………………………………………………………………………...….…ikepisi eliluhlaza

•I-substrate solution A 7ml…………………………………………………………………………………ikepisi elimhlophe

•I-substrateIsixazululo B 7ml ……………………………………………………………..…………………ikepisi elibomvu

• Isixazululo sokumisa 7ml ……………………………………………………………….………………………ikepisi eliphuzi

• 20×isixazululo se-Wash esigxilile esingu-40ml………..……………………………………….…...…ikepisi esobala

Ukuzwela, ukunemba nokunemba

Ukuzwela Kokuhlola: 0.4ppb

Umkhawulo wokutholwa

Okuphakelayo………………………………………………………….……………………………..5ppb

Ukunemba

Okuphakelayo………………………………………………………………….……….….…90±20%

Ukunemba

I-Coefficient ehlukile yekhithi ye-ELISA ingaphansi kuka-10%.

I-Cross Rate

I-Ochratoxin A………………………………………………………..………………..100%