chigadzirwa

About

Kiti iyi inogona kushandiswa mukuwongorora kwehuwandu uye hwemhando yeochratoxin A mukudya.Icho chigadzirwa chitsva chekuonekwa kwezvakasara zvezvinodhaka zvichibva patekinoroji yeELISA, inongodhura 30min mukushanda kwega kwega uye inogona kuderedza zvakanyanya kukanganisa kwekushanda uye kusimba kwekushanda.Iyi kit yakavakirwa pane isina kunanga inokwikwidza ELISA tekinoroji.Iyo microtiter matsime akaputirwa nekubatanidza antigen.Ochratoxin A mumuenzaniso inokwikwidza neantigen yakavharwa pa microtiter plate kune masoja ekudzivirira chirwere akawedzerwa.Mushure mekuwedzera kwe enzyme conjugate, TMB substrate inoshandiswa kuratidza ruvara.Absorbance yemuenzaniso haina hukama kune o chratoxin A yakasara mairi, mushure mekuenzanisa neiyo Standard Curve, yakapetwa ne dilution zvinhu,Ochratoxin Huwandu mumuenzaniso hunogona kuverengerwa.

Kit Zvikamu

• Microtiter plate ine 96 matsime akaputirwa neantigen

•Sakajairika mhinduro (6 mabhodhoro: 1ml/bhodhoro)

0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb

• Enzymeconjugate7ml……………………………………………………………………..………..…..chivharo chitsvuku

• Mushonga wekurwisa zvirwere10ml…………………………………………………………………………...….…green cap

•Substrate sotion A 7ml ………………………………………………………………………………

•SubstrateSolution B 7ml……………………………………………………………..…………………kapu dzvuku

• Stop solution 7ml ……………………………………………………………….……………………yellow cap

• 20×yakanyanya Wash mhinduro 40ml………..……………………………………….…...…transparent cap

Sensitivity, kururama uye nemazvo

Muedzo Sensitivity: 0.4ppb

Detection limit

Feed ………………………………………………………….………..……………….… 5ppb

Kururama

Feed ……………………………………………………………….……….….…90±20%

Precision

Kusiyanisa coefficient ye ELISA kit iri pasi pe10%.

Cross Rate

Ochratoxin A……………………………………………………..………………..100%