imveliso

Malunga

Le khithi ingasetyenziswa kuhlalutyo lobungakanani kunye nomgangatho we-ochratoxin A kwisondlo.Yimveliso entsha yokufunyanwa kwentsalela yechiza esekwe kwitekhnoloji ye-ELISA, exabisa kuphela i-30min kumsebenzi ngamnye kwaye inokunciphisa kakhulu iimpazamo zokusebenza kunye nokuqina komsebenzi.Le khithi isekelwe kubuchwephesha obungathanga ngqo be-ELISA.Imithombo ye-microtiter ifakwe kwi-antigen yokudibanisa.I-Ochratoxin A kwisampulu ikhuphisana ne-antigen egqunywe kwipleyiti ye-microtiter ye-antibody eyongeziweyo.Emva kokongezwa kwe-enzyme conjugate, i-substrate ye-TMB isetyenziselwa ukubonisa umbala.Ukungabikho kwesampulu kunxulumene kakubi ne-o chratoxin A intsalela kuyo, emva kokuthelekisa kunye ne-Standard Curve, iphindaphindwe yimiba yokuxutywa,Ochratoxin Ubungakanani kwisampulu ingabalwa.

Izixhobo zeKit

• I-Microtiter plate enemingxuma engama-96 eqatywe nge-antigen

•Sizisombululo eziqhelekileyo(iibhotile ezi-6:1ml/ibhotile)

0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb

• I-Enzymedibanisa7ml……………………………………………………………………..………..…..ikepusi ebomvu

• Isisombululo se-antibody10ml………………………………………………………………………...….…ikepusi eluhlaza

•Isubstrate solution A 7ml ……………………………………………………………………………

•Inxalenye engaphantsiIsisombululo B 7ml …………………………………………………………..…………………ikepusi ebomvu

• Misa isisombululo 7ml ……………………………………………………………….………………………ikepusi etyheli

• 20×isisombululo esigxininisiweyo seWash 40ml………..……………………………………….…...…umnqwazi ocacileyo

Uvakalelo, ukuchaneka kunye nokuchaneka

Uvakalelo loVavanyo: 0.4ppb

Umda wokufunyanwa

Ukutya ……………………………………………………….………..………………….… 5ppb

Ukuchaneka

Ukutya ……………………………………………………………….……….….…90±20%

Ukuchaneka

Ukwahluka komlinganiso wekhithi ye-ELISA ingaphantsi kwe-10%.

Inqanaba lokuwela

Ochratoxin A……………………………………………………..………………..100%