Online Exporter cell culture applications - Elisa Test Kit of AOZ – kwinbon
Online Exporter cell culture applications - Elisa Test Kit of AOZ – kwinbon Detail:
Nitrofurans are synthetic broad-spectrum antibiotics, which are frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties.
They had also been used as growth promoters in pig, poultry and aquatic production. In long term studies with lab animals indicated that the parent drugs and their metabolites showed carcinogenic and mutagenic characteristics. The nitrofuran drugs furaltadone, nitrofurantoin and nitrofurazone were banned from use in food animal production in the EU in 1993, and the use of furazolidone was prohibited in 1995.
Details
1.Elisa Test Kit of AOZ
2.Cat. A008-96 Wells
3.Kit Components
● Microtiter plate coated with antigen, 96 wells
● Standard solutions(6 bottles,1ml/bottle)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
● Spiking standard control : (1ml/bottle)................................................….100ppb
● Enzyme conjugate concentrate 1.5ml..............................................….….red cap
● Antibody solution concentrated 0.8ml……………………………....…green cap
● Substrate A 7ml……….........................................................…....…..…..white cap
● Substrate B7ml………..............….................................................…..…..red cap
● Stop solution 7ml……………………………………………….………yellow cap
● 20×concentrated wash solution 40ml ……………………….……transparent cap
● 2×concentrated extraction solution 60ml…………………..…………….blue cap
● 2-Nitrobenzaldehyde 15.1mg……………………………………………black cap
4.Sensitivity, accuracy and precision
Sensitivity: 0.025ppb
Detection limit……………..…………………………0.1ppb
Accuracy:
Animal tissue (muscle and liver)……….…………75±15%
Honey………………………………..………………..90±20%
Egg…………………………………..……………..…90±20%
Milk…………………………………..……………..…90±10%
Precision: CV of the ELISA kit is less than 10%.
5.Cross Rate
Furazolidone metabolite (AOZ)………………………….……………..100%
Furaltadone metabolite (AMOZ)………………………….……………<0.1%
Nitrofurantoin metabolite (AHD)………………………………………<0.1%
Nitrofurazone metabolite (SEM)…………………….……………...…<0.1%
Furazolidone………………………………………….………….…..…16.3%
Furaltadone……………………………………….………………….…<1%
Nitrofurantoin………………………………………………….…….…<1%
Nitrofurazone…………………………………….……………………..…<1%
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