Professional Design Pesticide Applicator Practice Test - Elisa Test Kit of Aflatoxin B1 – kwinbon
Professional Design Pesticide Applicator Practice Test - Elisa Test Kit of Aflatoxin B1 – kwinbon Detail:
Large doses of aflatoxins lead to acute poisoning (aflatoxicosis) that can be life threatening, usually through damage to the liver.
Aflatoxin B1 is an aflatoxin produced by Aspergillus flavus and A. parasiticus. It is a very potent carcinogen. This carcinogenic potency varies across species with some, such as rats and monkeys, seemingly much more susceptible than others. Aflatoxin B1 is a common contaminant in a variety of foods including peanuts, cottonseed meal, corn, and other grains; as well as animal feeds. Aflatoxin B1 is considered the most toxic aflatoxin and it is highly implicated in hepatocellular carcinoma (HCC) in humans.[citation needed] In animals, aflatoxin B1 has also been shown to be mutagenic, teratogenic, and to cause immunosuppression. Several sampling and analytical methods including thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), mass spectrometry, and enzyme-linked immunosorbent assay (ELISA), among others, have been used to test for aflatoxin B1 contamination in foods. According to the Food and Agriculture Organization (FAO), the worldwide maximum tolerated levels of aflatoxin B1 was reported to be in the range of 1–20 μg/kg in food, and 5–50 μg/kg in dietary cattle feed in 2003.
Details
1.Elisa Test Kit for Aflatoxin B1
2.Cat. KA07202H-96wells
3. Kit components
● Microtiter plateprecoated with antigen, 96 wells
● Standard Solution ×6bottle(1ml/bottle)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb,1.62ppb
● Enzyme conjugate 7ml………………………………………………………..…………red cap
● Antibody solution7ml..................................................................................………green cap
● Substrate A 7ml………………………………………………………….…………...…white cap
● Substrate B 7ml……………….……………………………………………….…………red cap
● Stop solution 7ml……….…….………………………………………………..………yellow cap
● 20×concentrated wash solution 40ml …………………………………………transparent cap
● 2×concentrated extraction solution 50ml………………………………………………blue cap
4.Sensitivity, accuracy and precision
Sensitivity: 0.05ppb
5.Detection limit
Edible oil sample.........................................................................................................................0.1ppb
Peanut..........................................................................................................................0.2ppb
Cereal.........................................................................................................................0.05ppb
Accuracy
Edible oil sample.....................................................................................................................80±15%
Peanut........................................................................................................................80±15%
Cereal........................................................................................................................80±15%
Precision: Variation coefficient of the ELISA kit is less than 10%.
6.Cross Rate
Aflatoxin B1·················100%
Aflatoxin B2·······················81 .3%
Aflatoxin G1·······················62%
Aflatoxin G2·······················22.3%
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By Diana from Slovakia - 2018.11.04 10:32
