ngwaahịa

Ihe gbasara

A na-eji ngwa a maka nyocha qualitative ngwa ngwa nke tetracyclines na nlele mmanụ aṅụ.

Usoro nkwadebe sample;

  (1) Ọ bụrụ na mmanụ aṅụ sample crystallized, kpoo ya na mmiri ịsa ahụ adịghị elu karịa 60 ℃, ruo mgbe mmanụ aṅụ sample mebie, mix kpamkpam, jụrụ dị ka ụlọ okpomọkụ, mgbe ahụ arọ maka assay.

(2) tụọ 1.0 ± 0.05g homogenate n'ime 10ml polystyrene centrifuge tube, tinye 3ml sample mmịpụta ngwọta, vortex maka 2min ma ọ bụ ịkwanye ya n'aka ruo mgbe sample-agwakọta kpamkpam.

Arụ ọrụ nyocha.

(1.) Were karama dị mkpa site na ngwugwu kit, wepụ kaadị achọrọ, ma mee akara kwesịrị ekwesị.Biko jiri kaadị ule ndị a n'ime 1h mgbe emepechara ngwugwu.

(2.) Were 100ml kwadebere sample n'ime sample oghere site pipette, wee malite ngụ oge mgbe mmiri mmiri eruba..

(3.) Tinye maka 10min na ụlọ okpomọkụ.

LOD

 Nsonaazụ

Enwere ahịrị abụọ na mpaghara nsonaazụ kaadị,Ahịrị njikwanaTetracylcines Line, nke e depụtara nkenke dị ka "B"na"T".Nsonaazụ ule ga-adabere na agba nke ahịrị ndị a.Eserese na-esonụ na-akọwa njirimara nsonaazụ.

Ihe ọjọọ: akara akara na ule ule abụọ na-acha uhie uhie na T Line dị ọchịchịrị karịa akara akara;

Tetracyclines dị mma: akara akara na-acha uhie uhie, T Line enweghị agba ma ọ bụ T Line dị ọkụ karịa ahịrị C, ma ọ bụ T Line bụ otu C Line.