ngwaahịa

Ihe gbasara

Enwere ike iji ngwa a mee ihe na nyocha nke quantitative na qualitative nke AMOZ fọdụrụnụ na ngwaahịa mmiri (azụ na oporo), wdg Enzyme immunoassays, ma e jiri ya tụnyere usoro chromatographic, na-egosi uru dị ukwuu banyere mmetụta uche, njedebe nchọpụta, akụrụngwa teknụzụ na oge achọrọ.

Emebere ngwa a iji chọpụta AMOZ dabere na ụkpụrụ nke immunoassay enzyme asọmpi na-apụtaghị ìhè.A na-ejikọta olulu mmiri microtiter na njide BSA

antigen.AMOZ na sample na-asọmpi na antigen mkpuchi na efere microtiter maka mgbochi agbakwunyere.Mgbe mgbakwunye nke enzyme conjugate, a na-eji mkpụrụ chromogenic eme ihe wee tụọ mgbama site na spectrophotometer.Mmụba a na-agbanwe agbanwe na ntinye AM OZ na nlele.

Ngwa ngwa

· Microtiter efere nwere olulu mmiri 96 nke ejiri antigen kpuchie

· Ihe ngwọta ọkọlọtọ ( karama 6)

0ppb, 0.05ppb,0.15ppb,0.45ppb,1.35ppb,4.05ppb

Ọkọlọtọ ngwọta na-efe efe: (1ml/ karama) …………………………………………………………………………100pb

Enzyme conjugate 1ml…………………………………………………………………………………………. okpu uhie

Ihe mgbochi mgbochi 7ml ………………………………………………………………………….…. okpu akwụkwọ ndụ akwụkwọ ndụ

Ihe ngwọta A 7ml……………………………………………………………………………………….…………………………. okpu ọcha

Ihe ngwọta B 7ml……………………………………………………………………………………….………………………. okpu uhie

ngwọta nkwụsị 7ml……………………………………………………………………………….………………. okpu edo edo

· 20× ndọpụ asacha ihe ngwọta 40ml……………………………………………………………………. transperent okpu

· 2× ihe ndọpụ uche mmịpụta 50ml………………………………………………………………………. okpu na-acha anụnụ anụnụ

2-Nitrobenzaldehyde 15.1mg………………………………………………………………………………………………

Mmetụta, izi ezi na nkenke

Mmetụta: 0.05pb

Oke nchọpụta

Ngwaahịa mmiri (azụ na oporo)……………………………… 0.1ppb

izi ezi

Ngwaahịa mmiri (azụ na oporo) ………………………………… 95± 25%

Nkenkenke:CV nke ngwa ELISA erughị 10%.

Ọnụọgụ gafere

Furaltadone metabolite (AMOZ) ………………………………………………….………… 100%

Furazolidone metabolite (AMOZ)..………………………………….<0.1%

Nitrofurantoin metabolite (AHD).………………… <0.1%

Nitrofurazone metabolite (SEM) ………………………………………………………………….... <0.1%

Furaltadone ………………………………………………………………………………………….…….11.1%

Furazolidone ………………………………………………………………………….………………… <0.1%

Nitrofurantoin………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………….

Nitrofurazone ………………………………………………………….………………………………………………………………………………………….. <1%