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Elisa Ikizamini cya AMOZ Igishusho cyihariye
  • Elisa Ikizamini cya AMOZ
  • Elisa Ikizamini cya AMOZ
  • Elisa Ikizamini cya AMOZ
  • Elisa Ikizamini cya AMOZ

Elisa Ikizamini cya AMOZ

Ibisobanuro bigufi:

Imiti ya nitrofuran furaltadone, nitrofurantoin na nitrofurazone yabujijwe gukoresha mu gutunganya amatungo y’ibiribwa mu muryango w’ubumwe bw’ibihugu by’Uburayi mu 1993, kandi byari bibujijwe gukoresha furazolidone mu 1995. Isesengura ry’ibisigazwa by’ibiyobyabwenge bya nitrofuran bigomba gushingira ku kumenya metabolite ihujwe na tissue. y'imiti y'ababyeyi ba nitrofuran, kubera ko imiti y'ababyeyi ihindagurika cyane, kandi tissue ihuza nitrofuran metabolite izagumana igihe kirekire, bityo metabolite ikoreshwa nk'intego mu gutahura ikoreshwa rya nitrofurani.Furazolidone metabolite (AMOZ), metabolite ya Furaltadone (AMOZ), metabolite ya Nitrofurantoin (AHD) na metabolite ya Nitrofurazone (SEM).

Injangwe.KA00205H-96 Iriba


Amabwiriza yo Gukoresha

Ibicuruzwa birambuye

Ibicuruzwa

Ibyerekeye

Iki gikoresho gishobora gukoreshwa mu isesengura ryinshi kandi ryujuje ubuziranenge bw’ibisigazwa bya AMOZ mu bicuruzwa byo mu mazi (amafi na shrimp), n’ibindi.

Iki gikoresho cyagenewe kumenya AMOZ gishingiye ku ihame rya enzyme irushanwa itaziguye immunoassay.Amariba ya microtiter yashizwemo no gufata BSA ihujwe

antigen.AMOZ murugero irushanwa na antigen yometse ku isahani ya microtiter ya antibody yongeyeho.Nyuma yo kongeramo enzyme conjugate, substrate ya chromogenic ikoreshwa kandi ikimenyetso gipimwa na spekitifotometero.Kwinjiza biringaniye na AM OZ yibanze muri sample.

Ibigize ibikoresho

Isahani ya Microtiter ifite amariba 96 yometse kuri antigen

· Ibisubizo bisanzwe (amacupa 6)

0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb

· Gutera igisubizo gisanzwe: (1ml / icupa) …………………………………………… ...100ppb

· Enzyme conjugate 1ml ………………………………………………… .. …………… .umutwe

· Antibody igisubizo 7ml ……………………………………………… .. ………….… .Icyatsi kibisi

· Igisubizo A 7ml …………………………………………………….Umutwe wera

· Igisubizo B 7ml ………………………………………………………….……… .. …… ingofero itukura

· Guhagarika igisubizo 7ml …………………………………………………………….Umutwe w'umuhondo

· 20 × yibanze yo gukaraba 40ml ……………………………………. Cap capa ibonerana

· 2 × igisubizo cyibanze cyo gukuramo 50ml ………………………………………. …… .umutwe wururimi

· 2-Nitrobenzaldehyde 15.1mg ………………………………………………….

Ibyiyumvo, ukuri kandi neza

Ibyiyumvo: 0.05ppb

Imipaka ntarengwa

Ibicuruzwa byo mu mazi (amafi na shrimp) …………. ……… 0.1ppb

Ukuri

Ibicuruzwa byo mu mazi (amafi na shrimp)….… ... ………… 95 ± 25%

Icyitonderwa:CV ya ELISA kit kiri munsi ya 10%.

Igipimo cy'umusaraba

Furaltadone metabolite (AMOZ) ………………………………….………… 100%

Furazolidone metabolite (AMOZ) …………………………..……………… .. <0.1%

Nitrofurantoin metabolite (AHD) ……………………………….…………… <0.1%

Nitrofurazone metabolite (SEM) ………………………………………….. << 0.1%

Furaltadone …………………………………………………………………..11.1%

Furazolidone ……………………………………………………….….… ..… <0.1%

Nitrofurantoin ………………………………………………………. …….… <1%

Nitrofurazone …………………………………………….……………… ..… <1%

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