ibicuruzwa

Chloramphenicol ni antibiyotike ikora neza, ikoreshwa cyane mu kuvura indwara zitandukanye zanduza inyamaswa, kandi igira ingaruka mbi kuri bagiteri zitandukanye zitera indwara.Ikibazo gikomeye hamwe na chloramphenicol.Chloramphenicol ifite uburozi bukomeye ningaruka mbi, zishobora kubuza imikorere ya hematopoietic yimikorere yamagufa yumuntu, bigatera anemia ya aplastique yumuntu, granular leukocytose, neonatal, syndrome de gray imburagihe nizindi ndwara, hamwe nubushuhe buke bwibisigazwa byibiyobyabwenge nabyo bishobora gutera indwara.Kubwibyo, ibisigazwa bya chloramphenicol mubiribwa byamatungo bibangamira cyane ubuzima bwabantu.Kubwibyo, byarabujijwe cyangwa bikoreshwa cyane muri EU na Amerika.

Kwinbon iki gikoresho nigicuruzwa gishya gishingiye kuri ELISA, cyihuta (50min gusa mugikorwa kimwe), cyoroshye, cyukuri kandi cyoroshye ugereranije nisesengura ryibikoresho bisanzwe, bityo rero birashobora kugabanya cyane amakosa yibikorwa nuburemere bwakazi.

Kwisubiraho

Chloramphenicol ………………………………… .. …… 100%

Chloramphenicol palmitate …………………………… <0.1%

Thiamphenicol ………………………………… .. …… .. <0.1%

Florfenicol ……………………………………………… <0.1%

Cetofenicol ……………………………………… .. …… <0.1%

Ibigize ibikoresho

Isahani ya Microtiter yometse kuri antigen, 96wells

Ibisubizo bisanzwe (6 × 1ml / icupa)

0ppb, 0.025ppb, 0.075ppb, 0.3ppb, 1.2ppb, 4.8ppb

Gutera igisubizo gisanzwe: (1ml / icupa) …….…100ppb

Enzyme yibanze ya conjugate 1ml….… ..… .....… ..….… ..….… ..….… ...... cap

Enzyme conjugate diluent 10ml….… ..….… .............….… ..….… ..….… ..… ..

Igisubizo A 7ml …………… .......................................... ........… ..…. ………… .umutwe wera

Igisubizo B 7ml ………………… ........................................ ......... …….… ....… .umutwe

Hagarika igisubizo 7ml …………… .......................................... ........ ……. …….… ..Umutwe wumuhondo

20 × Gukemura neza gukemura 40ml ……………………………………….… .Umutwe wuzuye

2 solution Gukuramo ibisubizo bikurura 50ml ............................................ ........... ingofero yubururu

Ibisubizo

1 Ijanisha

Indangagaciro zingana zo kwinjiza agaciro zabonetse kubipimo hamwe nicyitegererezo bigabanijwe nigiciro cyo kwinjiza cyambere (zeru zeru) kandi cyikubye 100%.Ibipimo bya zeru rero bikozwe bingana na 100% kandi indangagaciro zo kwinjiza zavuzwe ku ijanisha.

B ——ibisanzwe (cyangwa icyitegererezo)

B₀ ——Absorbance zeru zisanzwe

2 Umurongo usanzwe

Gushushanya umurongo usanzwe: fata agaciro ko kwinjiza agaciro nkibipimo y-axis, igice cya logarithmic yo kwibanda kumurongo wa CAP ibisubizo (ppb) nka x-axis.

CAP yibanze kuri buri sample (ppb), ishobora gusomwa uhereye kumurongo wa kalibrasi, igwizwa nibintu bihuye na Dilution ihuye na buri cyitegererezo cyakurikijwe, kandi intumbero nyayo yicyitegererezo iraboneka.

Nyamuneka menya:

Kubisesengura ryamakuru yibikoresho bya ELISA, hateguwe software idasanzwe, ishobora gutumizwa kubisabwa.