oloa

Chloramphenicol o se vailaʻau faʻamaʻi lautele lautele, e masani ona faʻaaogaina i togafitiga o faʻamaʻi pipisi eseese o manu, ma e iai sona aʻafiaga i luga o le tele o siama pathogenic.Fa'afitauli matuia ile toega ole chloramphenicol.Chloramphenicol ei ai aʻafiaga ogaoga ma aʻafiaga, lea e mafai ona faʻalavelaveina le gaioiga o le hematopoietic o le gaʻo ponaivi o le tagata, mafua ai le anemia aplastic tagata, leukocytosis granular, neonatal, maʻi faʻamaʻi vave ma isi faʻamaʻi, ma le maualalo o faʻamaʻi o vailaʻau faʻamaʻi e mafai foi ona faʻamaʻi ai faʻamaʻi.O le mea lea, o toega o le chloramphenicol i meaʻai a manu e matua lamatia ai le soifua maloloina o tagata.O le mea lea, ua faʻasaina pe faʻasaʻo le faʻaaogaina i EU ma US.

Kwinbon o lenei pusa o se oloa fou e faʻavae i luga o le ELISA, e vave (naʻo le 50min i le tasi taʻaloga), faigofie, saʻo ma maaleale pe a faʻatusatusa i suʻesuʻega meafaigaluega masani, ma o lea e mafai ai ona faʻaitiitia le sese o le gaioiga ma le malosi o galuega.

Fa'asagaga fa'asagatau

Chloramphenicol…………………………………………100%

Chloramphenicol palmitate……………………………………<0.1%

Thiamphenicol……………………………………………………..<0.1%

Florfenicol………………………………………………<0.1%

Cetofenicol…………………………………………………………<0.1%

Vaega Vaega

Microtiter ipu ua ufiufi i antigen, 96wells

Fofo masani (6×1ml/fagu)

0ppb,0.025ppb,0.075ppb,0.3ppb,1.2ppb,4.8ppb

Fofo masani fa'afefete: (1ml/fagu) …….…100ppb

Concentrated enzyme conjugate 1ml………………………………………………………………………………………..pulou manino

Enzyme conjugate diluent 10ml…………………………………………………………………………………………………..pulou manino

Fofo A 7ml…………………………………………………… .............................pulou paepae

Fofo B 7ml…………………………………………………… .....................pulou mumu

Taofi fofo 7ml…………………………………………………… ......................pulou samasama

20×Fofo fufulu fufulu 40ml……………………………………………..pulou manino

2×Fuli fa'aputu 50ml............................................ ..........pulou lanumoana

I'uga

1 pasene o le mitiia

O tau faʻatatau o le faʻaogaina o mea e maua mo faʻataʻitaʻiga ma faʻataʻitaʻiga e vaevaeina i le tau faʻafefe o le tulaga muamua (zero standard) ma faʻateleina i le 100%.Ole tulaga ole zero ua fa'apea ua tutusa ile 100% ma o tau o le absorbance o lo'o ta'ua i pasene.

B ——absorbance standard (poʻo faʻataʻitaʻiga)

B₀ ——absorbance zero standard

2 Pipi Fa'ata'atia

E tusi se pi'o fa'ata'atia: ave le aofa'i o tulaga fa'atatau e pei o le y-axis, semilogarithmic o le fa'atonuga o le CAP fa'ata'ita'iga fofo (ppb) e pei o le x-axis.

O le fa'atonuga o le CAP o fa'ata'ita'iga ta'itasi (ppb), e mafai ona faitau mai le pupuni fa'avasegaina, e fa'atele i le fa'atatau Fa'ailo fa'atatau o fa'ata'ita'iga ta'itasi e mulimulita'i, ma maua ai le fa'atonuga moni o fa'ata'ita'iga.

Faamolemole matau:

Mo suʻesuʻega faʻamaumauga o pusa ELISA, ua atiaʻe polokalama faʻapitoa, lea e mafai ona faʻatonu i luga ole talosaga.