sehlahisoa

Chloramphenicol ke lithibela-mafu tse sebetsang hantle tse pharalletseng, tse sebelisoang hangata kalafong ea mafu a fapaneng a tšoaetsanoang a liphoofolo, 'me li na le phello e thibelang libaktheria tse fapaneng tsa pathogenic.Bothata bo boholo ka masala a chloramphenicol.Chloramphenicol e na le chefo e tebileng le litla-morao, tse ka thibelang ts'ebetso ea hematopoietic ea moko oa motho, e bakang khaello ea mali ea motho, granular leukocytosis, neonatal, lefu la pele ho nako ea bohlooho le mafu a mang, 'me likhahla tse tlaase tsa masala a lithethefatsi li ka boela tsa baka mafu.Ka hona, masala a chloramphenicol lijong tsa liphoofolo a beha kotsi e kholo bophelong ba batho.Ka hona, e thibetsoe kapa e sebelisoa ka mokhoa o thibetsoeng ho EU le US.

Kwinbon Kit ena ke sehlahisoa se secha se ipapisitseng le ELISA, e potlakileng (50min feela ts'ebetsong e le 'ngoe), e bonolo, e nepahetse ebile e na le kutloelo-bohloko ha e bapisoa le tlhahlobo e tloaelehileng ea lisebelisoa, ka hona e ka fokotsa phoso ea ts'ebetso le matla a ts'ebetso haholo.

Liphetoho tse fapaneng

Chloramphenicol ……………………………………………… 100%

Chloramphenicol palmitate ……………………………<0.1%

Thiamphenicol ………………………………………………..<0.1%

Florfenicol …………………………………………………<0.1%

Cetofenicol …………………………………………………<0.1%

Likarolo tsa Kit

Letlapa la Microtiter le koahetsoeng ka antigen, 96wells

Litharollo tse tloaelehileng (6×1ml/botlolo)

0ppb,0.025ppb,0.075ppb,0.3ppb,1.2ppb,4.8ppb

Tharollo e tloaelehileng ea Spiking: (1ml/botlolo) …….…100ppb

Enzyme e kopaneng ea conjugate 1ml….…..……………..….…..….…..….…...... cap e hlakileng

Enzyme conjugate diluent 10ml….…..….……………………..…..….…..….…..…..transparent cap

Tharollo A 7ml …………………………………………… ..............….………….kepisi e tšoeu

Tharollo B 7ml ………………………………………………… ………………….kepisi e khubelu

Stop solution 7ml ……………………………………… ........…….…….…..kepisi e mosehla

20×Tharollo e matla ea ho hlatsoa 40ml…………………………………………….….

2×Tharollo e tebileng ea ho ntša 50ml ............................................ ...........kepisi e putsoa

Liphetho

1 Peresente ea ho monya

Litekanyetso tse tloaelehileng tsa litekanyetso tsa ho monya tse fumanoeng bakeng sa litekanyetso le lisampole li arotsoe ke boleng ba ho monya ba tekanyo ea pele (zero standard ) 'me e atolosoa ka 100%.Tekanyetso ea zero ka hona e etsoa hore e lekane le 100% mme litekanyetso tsa ho monya li qotsitsoe ka liphesente.

B - - absorbance standard (kapa sampole)

B₀ ——absorbance zero standard

2 Mokokotlo o Tloaelehileng

Ho etsa mokokotlo o tloaelehileng: nka boleng ba ho monya ba maemo joalo ka y-axis, semi logarithmic ea mahloriso a tharollo ea maemo a CAP (ppb) joalo ka x-axis.

Khokahano ea CAP ea sampole ka 'ngoe (ppb), e ka baloang ho tsoa lekhalong la calibration, e atolosoa ka ntlha e lumellanang ea Dilution ea sampole ka 'ngoe e lateloang, 'me ho fumanoa palo ea 'nete ea sampole.

Ka kopo hlokomela:

Bakeng sa tlhahlobo ea lintlha tsa lisebelisoa tsa ELISA, software e khethehileng e entsoe, e ka laeloang ka kopo.