alaabta

Chloramphenicol waa antibiyootig ballaadhan oo wax ku ool ah, oo caadi ahaan loo isticmaalo daaweynta cudurrada faafa ee kala duwan ee xayawaanka, waxayna leedahay saameyn ka hortag ah oo ku saabsan noocyada kala duwan ee bakteeriyada pathogenic.Dhibaato halis ah oo ka jirta haraaga chloramphenicol.Chloramphenicol waxay leedahay waxyeelo halis ah oo sun ah iyo waxyeelo, taas oo joojin karta shaqada hematopoietic ee dhuuxa lafta bini'aadamka, taasoo keenta dhiig-yaraanta bini'aadamka aplastic anemia, granular leukocytosis, neonatal, cillad cawlan oo dhicis ah iyo cuduro kale, iyo uruurinta hoose ee haraaga daroogada ayaa sidoo kale keeni karta cudur.Sidaa darteed, hadhaaga chloramphenicol ee cuntada xoolaha ayaa khatar weyn ku ah caafimaadka aadanaha.Sidaa darteed, waa laga mamnuucay ama si xaddidan ayaa looga isticmaalay EU iyo US.

Qalabkan Kwinbon waa badeeco cusub oo ku salaysan ELISA, kaas oo degdeg ah (kaliya 50min hal hawlgal), fudud, sax ah oo xasaasi ah marka la barbar dhigo falanqaynta qalabka caadiga ah, oo sidaas awgeed waxay si weyn u yaraynaysaa qaladka hawlgalka iyo xoojinta shaqada.

Falcelin-celin

Chloramphenicol………………………………………………………………………………………

Chloramphenicol palmitate………………………………………………………………………………….

Thiamphenicol………………………………………………………………. <0.1%

Florfenicol…………………………………………………………………………………

Cetofenicol……………………………………………………………………………….

Qaybaha Kit

saxan Microtiter ah oo lagu dahaadhay antigen, 96wells

Xalalka caadiga ah (6×1ml/dhalo)

0ppb,0.025ppb,0.075ppb,0.3ppb,1.2ppb,4.8ppb

Xalka caadiga ah ee kasbanaya: (1ml/ dhalo) ………….100ppb

Isku-xidhka enzyme-ka kooban 1ml…………………………………………………………………………………………

Enzyme conjugate diluent 10ml …………………………………………………………………………………….

Xalka A 7ml ………………………………………………………… …………………………………. Koofi cad

Xalka B 7ml ………………………………………………… …………………………. Koofi cas

Jooji xal 7ml ………………………………………………………… ......... Koofi huruud ah

20 × Xalka dhaqidda ee xoogga leh 40ml……………………………………….…. dabool hufan

2×Xalka soo saarista xooga leh 50ml.................................. ..........koofi buluug ah

Natiijooyinka

1 nuugista boqolkiiba

Qiimaha celceliska ee qiyamka nuugista ee loo helay jaangooyooyinka iyo shaybaarada waxaa loo qaybiyaa qiimaha nuugista ee heerka koowaad (heer eber) waxaana lagu dhuftey 100%.Heerarka eber ayaa sidaas laga dhigay 100% qiimaha nuugista waxaa lagu soo xigtay boqolkiiba.

B —— heerka nuugista (ama muunad)

B₀ -- nuugista heerka eber

2 Qalooca caadiga ah

Si loo sawiro qalooca caadiga ah: u qaado qiimaha nuugista ee jaangooyooyinka sida dhidibka y, semi logarithmic ee fiirsashada ee xalka halbeegyada CAP (ppb) sida dhidibka x.

Xoojinta CAP ee muunad kasta (ppb), kaas oo laga akhrin karo qalooca cabbirka, waxaa lagu dhuftaa qodobka u dhigma ee Dilution ee muunad kasta oo la raaco, waxaana la helayaa uruurinta dhabta ah ee muunadda.

Fadlan ogow:

Falanqaynta xogta xirmooyinka ELISA, software gaar ah ayaa la sameeyay, kaas oo la dalban karo haddii la codsado.